High-fidelity SaCas9 identified by directional screening in human cells

• Haihua Xie ,

  Contributed equally to this work with: Haihua Xie, Xianglian Ge

  Roles Data curation, Formal analysis, Investigation, Methodology, Software, Validation

  Affiliation School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China

  http://orcid.org/0000-0003-2588-4108

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• Xianglian Ge ,

  Contributed equally to this work with: Haihua Xie, Xianglian Ge

  Roles Data curation, Formal analysis, Investigation

  Affiliation School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China

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• Fayu Yang,

  Roles Data curation, Formal analysis, Investigation

  Affiliation School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China

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• Bang Wang,

  Roles Formal analysis, Software

  Affiliation School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China

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• Shuang Li,

  Roles Data curation, Formal analysis

  Affiliation Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

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• Jinzhi Duan,

  Roles Data curation, Formal analysis

  Affiliation National Institute of Biological Sciences, Beijing, China

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• Xiujuan Lv,

  Roles Data curation, Formal analysis

  Affiliation School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China

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• Congsheng Cheng,

  Roles Formal analysis

  Affiliations The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China, Raymond G. Perelman Center for Cellular and Molecular Therapeutics, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States of America

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• Zongming Song,

  Roles Formal analysis, Funding acquisition

  Affiliation School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China

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• Changbao Liu,

  Roles Formal analysis, Funding acquisition

  Affiliation The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China

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• Junzhao Zhao,

  Roles Formal analysis, Funding acquisition

  Affiliation The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China

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• Yu Zhang,

  Roles Formal analysis, Methodology

  Affiliation National Institute of Biological Sciences, Beijing, China

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• Jinyu Wu,

  Roles Formal analysis

  Affiliation Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China

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• Caixia Gao,

  Roles Formal analysis

  Affiliation State Key Laboratory of Plant Cell and Chromosome Engineering, and Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

  http://orcid.org/0000-0003-3169-8248

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• Jinwei Zhang ,

  Roles Formal analysis, Funding acquisition, Writing – original draft, Writing – review & editing

  * E-mail: jinwei.zhang@nih.gov (JZ); gufenguw@gmail.com (FG)

  Affiliation Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0002-2114-173X

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•  [ ... ],
• Feng Gu

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: jinwei.zhang@nih.gov (JZ); gufenguw@gmail.com (FG)

  Affiliation School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China

  http://orcid.org/0000-0002-9600-8407

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