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Open Access
Peer-reviewed
Methods and Resources
Methods and Resources report novel methods, substantial improvements to current methodologies, or informational datasets.
ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
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Jelmer Willems ,
Contributed equally to this work with: Jelmer Willems, Arthur P. H. de Jong
Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing
Affiliation Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands
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Arthur P. H. de Jong ,
Contributed equally to this work with: Jelmer Willems, Arthur P. H. de Jong
Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing
Affiliation Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands
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Nicky Scheefhals,
Roles Conceptualization, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing
Affiliation Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands
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Eline Mertens,
Roles Investigation
Affiliation Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands
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Lisa A. E. Catsburg,
Roles Investigation
Affiliation Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands
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Rogier B. Poorthuis,
Roles Investigation
Affiliation Department of Translational Neuroscience, UMC Utrecht Brain Center, Utrecht University, Utrecht, the Netherlands
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Fred de Winter,
Roles Resources
Affiliation Laboratory for Neuroregeneration, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, the Netherlands
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Joost Verhaagen,
Roles Resources
Affiliation Laboratory for Neuroregeneration, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, the Netherlands
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Frank J. Meye,
Roles Resources
Affiliation Department of Translational Neuroscience, UMC Utrecht Brain Center, Utrecht University, Utrecht, the Netherlands
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Harold D. MacGillavry
Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing
* E-mail: h.d.macgillavry@uu.nl
Affiliation Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands
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ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
- Jelmer Willems,
- Arthur P. H. de Jong,
- Nicky Scheefhals,
- Eline Mertens,
- Lisa A. E. Catsburg,
- Rogier B. Poorthuis,
- Fred de Winter,
- Joost Verhaagen,
- Frank J. Meye,
- Harold D. MacGillavry
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- Published: April 10, 2020
- https://doi.org/10.1371/journal.pbio.3000665
- See the preprint