Cryo-EM structures of the human PA200 and PA200-20S complex reveal regulation of proteasome gate opening and two PA200 apertures

• Hongxin Guan ,

  Contributed equally to this work with: Hongxin Guan, Youwang Wang, Ting Yu, Yini Huang

  Roles Funding acquisition, Methodology, Project administration, Writing – original draft, Writing – review & editing

  Affiliation The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

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• Youwang Wang ,

  Contributed equally to this work with: Hongxin Guan, Youwang Wang, Ting Yu, Yini Huang

  Roles Methodology, Project administration, Software, Writing – original draft, Writing – review & editing

  Affiliations National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, University of Chinese Academy of Sciences, Beijing, China

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• Ting Yu ,

  Contributed equally to this work with: Hongxin Guan, Youwang Wang, Ting Yu, Yini Huang

  Roles Methodology, Project administration, Software

  Affiliation The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

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• Yini Huang ,

  Contributed equally to this work with: Hongxin Guan, Youwang Wang, Ting Yu, Yini Huang

  Roles Methodology, Project administration

  Affiliations The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China, Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China

  ⨯
• Mianhuan Li,

  Roles Methodology, Software

  Affiliations The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China, Institute of Clinical Sciences, The First Affiliated Hospital of Jinan University, Guangzhou, China

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• Abdullah F. U. H. Saeed,

  Roles Software, Writing – original draft, Writing – review & editing

  Affiliation The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  http://orcid.org/0000-0003-3279-205X

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• Vanja Perčulija,

  Roles Methodology, Writing – review & editing

  Affiliation The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  http://orcid.org/0000-0003-1075-188X

  ⨯
• Daliang Li,

  Roles Methodology, Project administration

  Affiliation The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  ⨯
• Jia Xiao,

  Roles Methodology, Software

  Affiliations The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China, Institute of Clinical Sciences, The First Affiliated Hospital of Jinan University, Guangzhou, China

  ⨯
• Dongmei Wang,

  Roles Methodology, Project administration

  Affiliation The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  http://orcid.org/0000-0003-2632-6562

  ⨯
• Ping Zhu ,

  Roles Funding acquisition, Project administration, Software, Writing – review & editing

  * E-mail: ouyangsy@fjnu.edu.cn (SO); zhup@ibp.ac.cn (PZ)

  Affiliations National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, University of Chinese Academy of Sciences, Beijing, China

  http://orcid.org/0000-0003-2648-3170

  ⨯
• Songying Ouyang

  Roles Funding acquisition, Project administration, Validation, Writing – review & editing

  * E-mail: ouyangsy@fjnu.edu.cn (SO); zhup@ibp.ac.cn (PZ)

  Affiliations The Key Laboratory of Innate Immune Biology of Fujian Province, Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China, National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China

  http://orcid.org/0000-0002-1120-1524

  ⨯