Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease

• Weizhong Chen,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Writing – original draft, Writing – review & editing

  Affiliation School of Physical Science and Technology, ShanghaiTech University, Shanghai, China

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• Hongyuan Zhang,

  Roles Investigation

  Affiliation School of Physical Science and Technology, ShanghaiTech University, Shanghai, China

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• Yifei Zhang,

  Roles Investigation

  Affiliation School of Physical Science and Technology, ShanghaiTech University, Shanghai, China

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• Yu Wang,

  Roles Investigation

  Affiliation School of Physical Science and Technology, ShanghaiTech University, Shanghai, China

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• Jianhua Gan ,

  Roles Investigation, Validation, Writing – review & editing

  * E-mail: quanjiangji@shanghaitech.edu.cn (Q.J.); ganjhh@fudan.edu.cn (J.G.)

  Affiliation State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Shanghai Public Health Clinical Center, School of Life Sciences, Fudan University, Shanghai, China

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• Quanjiang Ji

  Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

  * E-mail: quanjiangji@shanghaitech.edu.cn (Q.J.); ganjhh@fudan.edu.cn (J.G.)

  Affiliation School of Physical Science and Technology, ShanghaiTech University, Shanghai, China

  http://orcid.org/0000-0002-2321-8462

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