High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation–associated versus preneurulation-associated fate patterning

• Mukul Tewary ,

  Contributed equally to this work with: Mukul Tewary, Dominika Dziedzicka

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Collaborative Program in Developmental Biology, University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada, Centre for Stem Cells & Regenerative Medicine, King's College London, London, United Kingdom

  ⨯
• Dominika Dziedzicka ,

  Contributed equally to this work with: Mukul Tewary, Dominika Dziedzicka

  Roles Data curation, Investigation, Methodology

  Affiliation Research Group Reproduction and Genetics, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium

  ⨯
• Joel Ostblom,

  Roles Data curation, Formal analysis, Investigation, Software, Visualization

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada

  http://orcid.org/0000-0003-0051-3239

  ⨯
• Laura Prochazka,

  Roles Data curation, Formal analysis

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada

  ⨯
• Nika Shakiba,

  Roles Data curation

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada

  ⨯
• Tiam Heydari,

  Roles Software

  Affiliations Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada

  http://orcid.org/0000-0003-0465-8312

  ⨯
• Daniel Aguilar-Hidalgo,

  Roles Software

  Affiliations Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada

  http://orcid.org/0000-0003-1366-9574

  ⨯
• Curtis Woodford,

  Roles Data curation

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada

  ⨯
• Elia Piccinini,

  Roles Data curation

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada

  ⨯
• David Becerra-Alonso,

  Roles Validation

  Affiliation Department of Quantitative Methods, Universidad Loyola Andalucia, Sevilla, Spain

  http://orcid.org/0000-0001-5174-7743

  ⨯
• Alice Vickers,

  Roles Data curation

  Affiliation Centre for Stem Cells & Regenerative Medicine, King's College London, London, United Kingdom

  http://orcid.org/0000-0001-9149-4744

  ⨯
• Blaise Louis,

  Roles Data curation

  Affiliation Centre for Stem Cells & Regenerative Medicine, King's College London, London, United Kingdom

  http://orcid.org/0000-0002-5289-2245

  ⨯
• Nafees Rahman,

  Roles Data curation

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada

  ⨯
• Davide Danovi,

  Roles Writing – review & editing

  Affiliation Centre for Stem Cells & Regenerative Medicine, King's College London, London, United Kingdom

  http://orcid.org/0000-0003-4119-5337

  ⨯
• Mieke Geens,

  Roles Writing – review & editing

  Affiliation Research Group Reproduction and Genetics, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium

  http://orcid.org/0000-0003-2849-1406

  ⨯
• Fiona M. Watt,

  Roles Funding acquisition, Supervision, Writing – review & editing

  Affiliation Centre for Stem Cells & Regenerative Medicine, King's College London, London, United Kingdom

  http://orcid.org/0000-0001-9151-5154

  ⨯
•  [ ... ],
• Peter W. Zandstra

  Roles Conceptualization, Funding acquisition, Resources, Supervision, Writing – review & editing

  * E-mail: peter.zandstra@ubc.ca

  Affiliations Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada, Collaborative Program in Developmental Biology, University of Toronto, Toronto, Ontario, Canada, Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada, Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada

  http://orcid.org/0000-0003-2356-942X

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