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DNA cleavage

Conversion of a telomere resolvase into a Cre-like site-specific recombinase

Shu Hui Huang, Mahrokh Balouchi, Kerri Kobryn

Enhanced thermal stability enables human mismatch-specific thymine–DNA glycosylase to catalyse futile DNA repair

Diana Manapkyzy, Botagoz Joldybayeva, [ ... ], Murat K. Saparbaev

Design and characterization of hyperactive mutants of the Agrobacterium tumefaciens telomere resolvase, TelA

Shu Hui Huang, Kayla Abrametz, Siobhan L. McGrath, Kerri Kobryn

The telomere resolvase, TelA, utilizes an underwound pre-cleavage intermediate to promote hairpin telomere formation

Mahrokh Balouchi, Shu Hui Huang, Siobhan L. McGrath, Kerri Kobryn

Cas9 is mostly orthogonal to human systems of DNA break sensing and repair

Ekaterina A. Maltseva, Inna A. Vasil’eva, [ ... ], Olga I. Lavrik

A programmable DNA roadblock system using dCas9 and multivalent target sites

Emily K. Matozel, Stephen Parziale, Allen C. Price

Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing

Eric A. Noel, Donald P. Weeks, James L. Van Etten

A single-molecule counting approach for convenient and ultrasensitive measurement of restriction digest efficiencies

Yi Zhang, Takuro Nunoura, [ ... ], Shigeru Deguchi

DNA binding activity of the proximal C-terminal domain of rat DNA topoisomerase IIβ is involved in ICRF-193-induced closed-clamp formation

Shinji Kawano, Kunpei Fujimoto, Kazushi Yasuda, Shogo Ikeda

A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining

Danielle Guillen, Mika Schievelbein, [ ... ], Jonathan Ouellet

Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system

Anders W. Matson, Nora Hosny, [ ... ], Christopher Burlak

Epstein-Barr virus genome packaging factors accumulate in BMRF1-cores within viral replication compartments

Atsuko Sugimoto, Yoriko Yamashita, [ ... ], Tatsuya Tsurumi

Petri net–based model of the human DNA base excision repair pathway

Marcin Radom, Magdalena A. Machnicka, [ ... ], Piotr Formanowicz

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