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Cloning

TReSR: A PCR-compatible DNA sequence design method for engineering proteins containing tandem repeats

James A. Davey, Natalie K. Goto

published 12 Apr 2023

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Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity

Roxana Portieles, Hongli Xu, Feng Chen, Jingyao Gao, Lihua Du, Xiangyou Gao, Carlos Borroto Nordelo, Qiulin Yue, Lin Zhao, Nayanci Portal Gonzalez, Ramon Santos Bermudez, Orlando Borrás-Hidalgo

published 10 Apr 2023

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The selection of software and database for metagenomics sequence analysis impacts the outcome of microbial profiling and pathogen detection

Ruijie Xu, Sreekumari Rajeev, Liliana C. M. Salvador

published 07 Apr 2023

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Issues with RNF43 antibodies to reliably detect intracellular location

Shanshan Li, Ruyi Zhang, Marla Lavrijsen, Thierry P. P. van den Bosch, Maikel P. Peppelenbosch, Ron Smits

published 06 Apr 2023

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Maternal effect in salinity tolerance of Daphnia–One species, various patterns?

Andrzej Mikulski, Danuta Mazurczak

published 04 Apr 2023

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A real-time voice cloning system with multiple algorithms for speech quality improvement

Weixin Hu, Xianyou Zhu

published 03 Apr 2023

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Increased levels of immature and activated low density granulocytes and altered degradation of neutrophil extracellular traps in granulomatosis with polyangiitis

Spyridon Lipka, Lennard Ostendorf, Udo Schneider, Falk Hiepe, Falko Apel, Tobias Alexander

published 15 Mar 2023

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Clonal isolates of Treponema pallidum subsp. pallidum Nichols provide evidence for the occurrence of microevolution during experimental rabbit infection and in vitro culture

Diane G. Edmondson, Bridget D. De Lay, Blake M. Hanson, Lindsay E. Kowis, Steven J. Norris

published 14 Mar 2023

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Standardization of flow cytometry and cell sorting to enable a transcriptomic analysis in a multi-site sarcoidosis study

Roman E. Magallon, Laura D. Harmacek, Nicholas K. Arger, Pineet Grewal, Linda Powers, Brenda R. Werner, Briana Q. Barkes, Li Li, Kristyn MacPhail, May Gillespie, Elizabeth K. White, Sarah E. Collins, Talyor Brown, Jessica Cardenas, Edward S. Chen, Lisa A. Maier, Sonia M. Leach, Nabeel Y. Hamzeh, Laura L. Koth, Brian P. O’Connor

published 09 Mar 2023

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Direct selection of functional fluorescent-protein antibody fusions by yeast display

Nileena Velappan, Fortunato Ferrara, Sara D’Angelo, Devin Close, Leslie Naranjo, Madeline R. Bolding, Sarah C. Mozden, Camille B. Troup, Donna K. McCullough, Analyssa Gomez, Marijo Kedge, Andrew R. M. Bradbury

published 24 Feb 2023

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Imiquimod induces skin inflammation in humanized BRGSF mice with limited human immune cell activity

Pernille Kristine Fisker Christensen, Axel Kornerup Hansen, Søren Skov, Britta Cathrina Martel, Jesper Larsen, Maria Helena Høyer-Hansen, Janne Koch

published 17 Feb 2023

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In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1

Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran

published 16 Feb 2023

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Combining vertebrate mitochondrial 12S rRNA gene sequencing and shotgun metagenomic sequencing to investigate the diet of the leopard cat (Prionailurus bengalensis) in Korea

Cheolwoon Woo, Priyanka Kumari, Kyung Yeon Eo, Woo-Shin Lee, Junpei Kimura, Naomichi Yamamoto

published 31 Jan 2023

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