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Vector cloning

Superoxide dismutase VPA1514 in Vibrio parahaemolyticus protects against environmental stresses

Paula Hsu, Hin-chung Wong, Chung-Tao Tang

Different results despite high homology: Comparative expression of human and murine DNase1 in Pichia pastoris

Jan-Ole Krischek, Hans Georg Mannherz, Markus Napirei

Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

Pradip Kumar Singh, Michael S. Donnenberg

DAPE cloning with modified primers for producing designated lengths of 3’ single-stranded ends in PCR products

Seoee Lee, Hyunyoung Kim, [ ... ], Hyunju Ro

Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro

Keishi Shintomi, Yuki Masahara-Negishi, [ ... ], Tatsuya Hirano

A comprehensive, improved protocol for generating common bean (Phaseolus vulgaris L.) transgenic hairy roots and their use in reverse-genetics studies

Ronal Pacheco, Georgina Estrada-Navarrete, [ ... ], Carmen Quinto

A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes

Shaun Ferguson, Nikolaj B. Abel, [ ... ], Simona Radutoiu

The chicken chorioallantoic membrane model for isolation of CRISPR/cas9-based HSV-1 mutant expressing tumor suppressor p53

Mishar Kelishadi, Hosein Shahsavarani, [ ... ], Kayhan Azadmanesh

Improved USER cloning for TALE assembly and its application to base editing

Jizeng Zhou, Jiaowei Wang, [ ... ], Qingjian Zou

High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, [ ... ], Dan Su

Characterization of an intracellular humanized single-chain antibody to matrix protein (M1) of H5N1 virus

He Sun, Guangmou Wu, [ ... ], Yuan Tian

MICA-G129R: A bifunctional fusion protein increases PRLR-positive breast cancer cell death in co-culture with natural killer cells

Hui Ding, Garrett W. Buzzard, [ ... ], Yanzhang Wei

A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

Korbinian N. Kropp, Tim J. Schäufele, [ ... ], Catherine Wölfel

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