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Gene cloning

pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2

Inês M. Luís, Mariana Parada, João B. Vicente, Isabel A. Abreu

Susceptibility profile of Anopheles and target site resistance mechanism against organophosphates in Cameroon.

Judith Dandi-Labou, Jonas A. Kengne-Ouafo, [ ... ], Charles S. Wondji

Transcriptomic analysis of benznidazole-resistant Trypanosoma cruzi clone reveals nitroreductase I-independent resistance mechanisms

Ana María Mejía-Jaramillo, Hader Ospina-Zapata, Geysson Javier Fernandez, Omar Triana-Chávez

Evidence for gene essentiality in Leishmania using CRISPR

Wen-Wei Zhang, Greg Matlashewski

A cell competition system with one gene expression from a single-copy gene in one cell

Yoshinori Hasegawa, Megumi Nakano, [ ... ], Osamu Ohara

Unraveling the impact of AXIN1 mutations on HCC development: Insights from CRISPR/Cas9 repaired AXIN1-mutant liver cancer cell lines

Ruyi Zhang, Shanshan Li, [ ... ], Ron Smits

HMGR and CHS gene cloning, characterizations and tissue-specific expressions in Polygala tenuifolia Willd

Yang Liu, Xiaofang Ma, [ ... ], Yunsheng Zhao

Generation of murine tumor models refractory to αPD-1/-L1 therapies due to defects in antigen processing/presentation or IFNγ signaling using CRISPR/Cas9

Paul L. Chariou, Christine M. Minnar, [ ... ], Sofia R. Gameiro

Resistome and virulome of high-risk pandemic clones of multidrug-resistant extra-intestinal pathogenic Escherichia coli (ExPEC) isolated from tertiary healthcare settings in Uganda

Denis K. Byarugaba, Bernard Erima, [ ... ], Fred Wabwire-Mangen

Functional characterization of a single nucleotide polymorphism associated with Alzheimer’s disease in a hiPSC-based neuron model

Lindsay R. Stolzenburg, Sahar Esmaeeli, [ ... ], Cyril Y. Ramathal

Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity

Roxana Portieles, Hongli Xu, [ ... ], Orlando Borrás-Hidalgo

In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1

Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran

High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, [ ... ], Dan Su

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