Toll signaling in fat body acts in a tissue-autonomous manner to disrupt nutrient storage

Genetic activation of Toll signaling in the larval fat body, via r4-GAL4-dependent expression of a constitutively-active Toll^10b transgene, reduces whole-animal triglyceride levels, although the mechanism for reduced lipid storage has been unclear [24,25]. The fat body stores the bulk of triglycerides in fruit fly larvae, and we find that activating Toll signaling in larval fat body, via r4-GAL4 driven expression of Toll^10b, decreased late third instar fat body triglyceride levels by 55%. Expression of Toll^10b in fat body led to negligible changes in gut and carcass triglycerides compared with tissues from control larvae expressing GFP in fat body (Fig 1A). We also note, as expected, that the fat body stored 70–85 times more triglyceride than the gut or the carcass, which comprises cuticle, muscle, oenocytes, imaginal discs, and trachea. Throughout the larval third instar, animals with active fat body Toll signaling consistently stored less triglyceride than GFP-expressing controls (S1 Fig). Reduced triglyceride storage in animals with active Toll signaling persisted to the white prepupal stage, a well-defined developmental endpoint that follows the cessation of feeding, indicating that low triglyceride levels are not due to a developmental delay. To understand whether the decrease in triglycerides elicited by innate immune signaling is transcriptionally regulated, we manipulated the NF-κB homolog Dif. Dif is activated by Toll signaling and binds directly to promoters of genes encoding antimicrobial peptides (AMPs) such as Drosomycin, leading to high levels of AMP production in fat body in response to infection with fungi or Gram-positive bacteria such as Enterococcus faecalis (S2 Fig). Elevated expression of Dif in larval fat body phenocopied Toll^10b, leading to a decrease in whole-animal triglyceride levels (Fig 1B). However, loss of Dif in fat bodies with active Toll signaling rescued triglyceride storage (Fig 1C).

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Fig 1. Toll signaling in the third instar larval fat body reduces triglyceride storage in a tissue-autonomous manner.

For A-C, r4-GAL4 was used to drive indicated transgenes in fat body, and triglyceride levels were measured in whole larvae or dissected organs and normalized to protein levels. (A) Triglyceride levels in fat body, gut, or carcass, n = 7-8/group. *p = 0.0118 and **p = 0.0047 versus GFP. (B) Whole-animal triglyceride levels in larvae expressing GFP or Dif in fat body, n = 18-20/group. **p = 0.0034 versus GFP. (C) Whole-animal triglyceride levels in larvae expressing GFP or Toll^10b+Dif^RNAi in fat body, n = 8/group. (D-F) Top: whole-animal triglyceride levels, normalized to protein, in controls and larvae subjected to sterile injury or injury with E. faecalis infection. Bottom: Western blot analysis of DrsGFP reporter levels in triglyceride lysates from larvae infected with E. faecalis. Histone H3 (HisH3) is shown as a loading control. (D) 24h post infection, n = 8-9/group. (E) 36h post infection, n = 9-16/group. *p = 0.0393. (F) 48h post infection, n = 14-17/group. Data are presented as means ± SD. p values were determined by Student’s unpaired t test (A-C) and one-way ANOVA with Dunnett’s multiple comparison test (D-F).

https://doi.org/10.1371/journal.pgen.1009192.g001

We used a second fat body driver, cg-GAL4, to validate our results with r4-GAL4. The r4-GAL4 and cg-GAL4 drivers induce UAS transgene expression in larval fat body, and each exhibits restricted expression in other organs (S3A Fig). Expression of Toll^10b under control of cg-GAL4 induces fat body expression of the antimicrobial peptide gene Drosomycin (S3B Fig) and lowers whole-animal triglyceride levels by 41% compared with GFP controls (S3C Fig).

To determine whether infection disrupts lipid storage in a manner similar to genetic activation of Toll signaling, we challenged early third instar larvae with the Gram-positive, pathogenic bacteria Enterococcus faecalis [27] and monitored triglyceride levels beginning at 24 hours after infection. We chose this time course because larval triglyceride storage increases three- to four-fold from the beginning of the third instar at 72h after egg lay (AEL) to the end of this stage 48 hours later (refer back to S1 Fig). Because injury also activates the innate immune response in a manner dependent on Toll pathway components including MyD88 [28,29], we included mock-infected controls that were punctured without bacterial exposure. At 24 hours post infection, triglyceride levels were equivalent among control larvae, larvae with sterile injury, and larvae with injury and bacterial infection (Fig 1D). At 36 hours post infection, control animals had doubled the level of stored triglyceride relative to the 24 hour time point (equivalent to 96h AEL). However, triglyceride accumulation was significantly reduced by 14% in infected larvae. Triglyceride levels were similarly decreased in larvae subjected to sterile injury, but this difference failed to reach statistical significance (p = 0.0701) (Fig 1E). By 48 hours post infection, triglyceride levels were again equivalent among groups, indicating a transient block of triglyceride accumulation in response to infection (Fig 1F). Larvae in this experiment carried DrsGFP, a reporter of Toll signaling consisting of the Drosomycin promoter fused to the GFP coding sequence [30]. Western analysis of the whole-animal lysates used for triglyceride measurements showed that at both 24 and 36 hours post infection, animals with the lowest triglyceride levels had the highest levels of DrsGFP expression (bottom panels, Fig 1D and 1E). By 48 hours post infection, when triglyceride levels were equivalent with control animals, there was no clear relationship between lipid storage and DrsGFP expression (bottom panel, Fig 1F).

A major role of stored triglycerides is to provide energy during starvation. We asked whether decreased triglyceride storage caused by larval fat body Toll signaling affected starvation resistance in adult flies that expressed GFP or Toll^10b in fat body throughout the larval and pupal stages under control of r4-GAL4. We saw no difference in starvation sensitivity in female or male flies of either genotype that were given water (S4A Fig). However, when we subjected flies to the combined stressor of starvation and desiccation, we observed a significant decrease in stress resistance in flies that expressed Toll^10b in fat body compared with GFP-expressing controls (S4B Fig). Median survival time during starvation without water was decreased by 33% and 43%, respectively, in male and female flies expressing Toll^10b compared with GFP-expressing controls. Decreased resistance to desiccation is consistent with a failure to waterproof the cuticle, and recent work shows that about half of the triglyceride stored by the larval fat body is used for this purpose [31].

We investigated the mechanism for reduced triglyceride storage in the immune-activated fat body by examining levels of circulating glucose, the substrate for de novo fatty acid and triglyceride synthesis. Trehalose, a disaccharide composed of two glucose molecules, is the major circulating sugar in fruit flies. Hemolymph trehalose and glucose levels were equivalent in larvae expressing GFP or Toll^10b in fat body (Fig 2A), suggesting that altered substrate availability does not account for reduced triglyceride storage. Another fate of glucose is storage as the branched polysaccharide glycogen. Surprisingly, whole-animal glycogen levels were increased in third instar larvae with active Toll signaling in fat body (Fig 2B). To better understand this phenotype, we measured glycogen in two tissues that are the major sites of glycogen storage in fly larvae, fat body and body wall muscles [32]. We observed a 3.7-fold increase in glycogen levels in fat body but no change in glycogen in the carcass, containing the body wall musculature, in animals with active fat body Toll signaling compared with controls (Fig 2C). Glycogen levels in white prepupae were reduced from third instar levels and were equivalent in animals expressing GFP or Toll^10b in fat body (Fig 2D). Together these data show that triglyceride and glycogen storage are regulated differently by innate immune signaling and that an impairment in lipid storage correlates with reduced survival in response to desiccation stress in the adult stage. Because genetic or physiological activation of the Toll pathway lowers triglyceride storage, our next step was to determine the molecular mechanisms underlying this phenotype.

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Fig 2. Toll signaling leads to increased fat body glycogen storage in the larval stage.

(A) Hemolymph glucose and trehalose levels in mid-third instar larvae expressing GFP or Toll^10b in fat body under control of r4-GAL4, n = 4-5/group. (B) Late third instar whole-animal glycogen levels, normalized to protein, n = 12/group. ***p = 0.0002 versus GFP. (C) Glycogen levels in fat body and body wall, normalized to protein, from late third instar larvae expressing GFP or Toll^10b in fat body, n = 11-13/group, ****p < 0.0001 versus GFP. (D) White prepupal whole-animal glycogen levels, normalized to protein, n = 14-17/group. Data are presented as means ± SD. p values were determined by Student’s unpaired t test.

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Toll signaling negatively regulates dedicated steps of triglyceride synthesis

De novo lipogenesis is controlled in part by transcriptional regulation of genes encoding lipogenic enzymes [33]. The enzymes ATP citrate lyase (ATPCL), Acetyl-CoA carboxylase (ACC), and Fatty acid synthase 1 (FASN1) synthesize fatty acids from glucose-derived citrate (Fig 3A). Fat body-specific expression of ATPCL was unchanged by activation of Toll signaling. Transcripts encoding ACC and FASN1 were elevated by 51–75% in Toll^10b-expressing fat bodies compared with controls, but these differences were not statistically significant (Fig 3B). In contrast, Toll signaling in fat body, induced by transgenic expression of Toll^10b with r4-GAL4 or cg-GAL4 led to a 39–45% reduction in transcripts encoding Lipin, a phosphatidic acid phosphatase that synthesizes diacylglycerol from phosphatidic acid (Figs 3C and S3D). However, unlike the results with chronic, high-level signaling induced by Toll^10b transgene expression, physiological activation of Toll signaling did not reduce Lipin mRNA levels after sterile injury or infection (Fig 3D).

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Fig 3. Reduced expression of Lipin and the DGAT homolog midway correlate with low triglyceride levels in larvae with active Toll signaling.

(A) Schematic representation of the de novo lipogenic pathway leading to triglyceride synthesis. (B-F) Total RNA was extracted from late third instar larval fat bodies expressing the indicated transgenes under control of r4-GAL4 or fat bodies from control, injured, and infected larvae dissected at indicated times following sterile injury or infection with Enterococcus faecalis early in the third instar stage. All transcript levels were measured by RT-qPCR and normalized to Rp49. (B) ATPCL, ACC, and FASN1 transcripts in late third instar larval fat bodies expressing GFP or Toll^10b, n = 7/group. (C) Lipin mRNA levels in late third instar larval fat bodies expressing GFP or Toll^10b, n = 7/group. *p = 0.0259 versus GFP. (D) Lipin mRNA levels in third instar larval fat bodies at 6–36 hours post infection, n = 7-10/group. *p ≤ 0.0133 versus uninfected controls. (E) midway mRNA levels in late third instar larval fat bodies expressing GFP or Toll^10b, n = 7/group. **p = 0.0031 versus GFP. (F) midway mRNA levels in third instar larval fat bodies at 6–36 hours post infection, n = 7-10/group. *p ≤ 0.0350, **p ≤ 0.0017, and ****p < 0.0001 versus uninfected controls. (G) Lipin activity in fat bodies from late third instar larvae expressing GFP or Toll^10b (left) and RFP+GFP, RFP+Lipin, or Toll^10b+Lipin (right) under control of r4-GAL4, n = 4-7/group. *p ≤ 0.0179 versus GFP (left) or RFP+GFP (right) and **p = 0.0097 versus RFP+GFP. (H) Triglyceride levels in late third instar larvae expressing Toll^10b with or without a wild type Lipin transgene in fat body, n = 8/group. *p = 0.0164 and **p = 0.0053 versus RFP+GFP. (I) Triglyceride levels in late third instar larvae expressing Toll^10b with or without a wild type mdy^HA transgene in fat body, n = 5-6/group. *p = 0.0193 and **p = 0.0020 versus RFP+GFP. (J) Triglyceride levels in late third instar larvae expressing Toll^10b with or without wild type Lipin and mdy^HA transgenes in fat body, n = 22-25/group. **p = 0.0016 and ****p < 0.0001 versus RFP+GFP, and *p = 0.0193 versus GFP+Toll^10b. Data are shown as means ± SD. p values were determined by Student’s t test (B, C, E and G, left panel) and one-way ANOVA with Dunnett’s (D, F, H, I) or the Tukey-Kramer (G, right panel, J) multiple comparisons test.

https://doi.org/10.1371/journal.pgen.1009192.g003

Genetic activation of fat body Toll signaling reduced expression of the diacylglycerol acyltransferase (DGAT) homolog midway, an enzyme that carries out the final step in triglyceride synthesis, by 38% compared with controls (Fig 3E). In contrast, we saw no change in midway expression when Toll^10b was expressed under cg-GAL4 control (S3D Fig). In response to sterile injury or infection with Enterococcus faecalis, however, fat body levels of midway were sharply reduced early and this decrease persisted for 24 hours following infection (Fig 3F).

Lipin and midway are necessary for triglyceride storage. Knockdown of either enzyme in fat body or loss of midway in the whole animal decreased triglyceride levels (S5A–S5C Fig), consistent with previous findings [34–37]. Elevated fat body expression of Lipin or midway was not sufficient to increase triglyceride accumulation in otherwise wild type larvae (S5D–S5F Fig). However, fat body-specific expression of a UAS-midway transgene restored triglycerides in flies heterozygous for the mdy^QX25 null mutation (S5G Fig).

We performed transgenic rescue experiments to determine whether forced expression of Lipin or midway would restore triglyceride accumulation in animals with active fat body Toll signaling. Consistent with decreased Lipin transcript levels, we find a 20% decrease in Lipin activity in extracts of fat bodies expressing Toll^10b compared with controls expressing GFP (Fig 3G, left). Forced expression of a wild type Lipin transgene in fat body increased Lipin activity 2.6-fold in extracts from fat bodies co-expressing RFP or Toll^10b compared with control extracts (Fig 3G, right). However, elevated Lipin expression and enzyme activity failed to rescue low triglyceride levels in larvae co-expressing Toll^10b (Fig 3H). The ability of Lipin overexpression to rescue Lipin activity but not triglyceride storage raised the possibility that midway was limiting for triglyceride storage during chronic Toll activation in the fat body. However, similar to results found with Lipin, co-expression of a wild type midway transgene with Toll^10b did not rescue triglyceride levels (Fig 3I).

We considered the possibility that when either Lipin or midway is overexpressed, the other enzyme becomes rate limiting in de novo lipogenesis. To test this, we generated flies co-expressing both Lipin and midway under control of the r4-GAL4 fat body driver, leading to 10-50-fold higher transcript levels of each enzyme even in the presence of Toll^10b (S5H Fig). Co-expression of Lipin and midway in fat body does not increase whole-animal triglyceride levels compared with controls expressing RFP and GFP (Fig 3J). In contrast, co-expression of Lipin and midway with Toll^10b in fat body led to a significant, 16% increase in triglyceride levels compared with animals expressing Toll^10b alone (Fig 3J). The failure of Lipin and midway co-expression to fully rescue triglyceride levels is consistent with the possibility that fatty acids are being diverted to another purpose in cells with active Toll signaling and that elevated flux through this second pathway could prevent triglyceride accumulation.

Innate immune signaling induces phospholipid synthesis

Given that ATPCL, ACC, and FASN1 are expressed at normal to elevated levels in fat bodies with active Toll signaling, we considered that fatty acids produced by the actions of these enzymes might be used for purposes other than triglyceride storage during the immune response. A major fate of fatty acids in cells is phospholipid synthesis via the Kennedy pathway (Fig 4A). We assessed transcript levels of Kennedy pathway homologs in fat bodies expressing GFP or Toll^10b under control of r4-GAL4 or cg-GAL4. We find elevated transcript levels of both the ethanolamine kinase easily shocked (eas) and the diacylglycerol ethanolaminephosphotransferase CG7149 in fat bodies with active Toll signaling compared with controls (Figs 4B and S3E). Phosphocholine cytidylyltransferase 1 (Pcyt1) was also induced by Toll signaling (Figs 4C and S3E), while the choline kinase homolog CG2201 was inconsistently increased by fat body Toll pathway activity (compare data from independent experiments shown in Figs 4C and S6A). Protein levels of eas and Pcyt1 were noticeably induced in fat bodies with active Toll signaling (Figs 4D, 4E and S3F). In response to sterile injury or infection with Enterococcus faecalis, fat body levels of eas were largely unchanged, although we note decreased levels in both groups at 6 hours post infection (Fig 4F). However, Pcyt1 transcript levels were induced in fat bodies during the first 18 hours following sterile injury or infection and then decreased to uninfected levels (Fig 4G). Other enzymes in the Kennedy pathway, such as Phosphoethanolamine cytidylyltransferase (Pect), the diacylglycerol cholinephosphotransferase bb in a boxcar (bbc) (Fig 4B and 4C), Phosphocholine cytidylyltransferase 2 (Pcyt2) and the diacylglycerol ethanolaminephosphotransferase CG33116 (S6B Fig) were not induced by Toll signaling. We note that our previously published RNA-Seq data [26] show much higher expression of Pcyt1 compared with Pcyt2 in larval fat body (S6C Fig).

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Fig 4. Toll signaling in fat body leads to increased levels of phospholipid synthesis enzymes and elevated membrane phospholipids.

(A) Schematic representation of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) synthesis via the Kennedy pathway. (B) Late third instar fat body levels of eas, Pect, and CG7149 transcripts, normalized to Rp49, n = 7/group. ***p = 0.0002 and ****p = 0.0001 versus GFP. (C) Late third instar fat body levels of CG2201, Pcyt1, and bbc transcripts, normalized to Rp49. n = 7/group. **p ≤ 0.0071 versus GFP. (D,E) Western blot analysis of eas (D) and Pcyt1 (E) protein levels in fat bodies expressing GFP or Toll^10b under control of r4-GAL4. Histone H3 levels are shown as loading controls. (F,G) eas (F) and Pcyt1 (G) mRNA levels, normalized to Rp49, in third instar larval fat bodies from uninfected controls, larvae subjected to sterile injury, and larvae infected with Enterococcus faecalis at 6–36 hours post infection, n = 7-10/group. *p ≤ 0.0496, **p ≤ 0.0035, and ***p = 0.0009 versus uninfected controls. (H) Mass spectrometry analysis of total phosphatidylethanolamine levels (PE, left) and phosphatidylcholine levels (PC, right) in lipid extracts from late third instar larval fat bodies expressing GFP or Toll^10b, n = 6/group. *p = 0.0417 and **p = 0.0080 versus GFP. (I) Mass spectrometry analysis of PE (left) and PC species (right) in lipid extracts from late third instar larval fat bodies expressing GFP or Toll^10b, n = 6/group. *p < 0.05, **p < 0.01 versus GFP. Data are shown as means ± SD. p values were determined by Student’s unpaired t test (B, C, H, I) and one-way ANOVA with Dunnett’s multiple comparisons test (F, G).

https://doi.org/10.1371/journal.pgen.1009192.g004

To determine the functional consequences of increased eas, CG7149, CG2201, and Pcyt1 expression in fat bodies with active Toll signaling, we assessed phospholipid levels in fat body. Using thin layer chromatography (TLC), we found increases in total phosphatidylethanolamine (PE) and phosphatidylcholine (PC) in fat bodies expressing Toll^10b compared with controls expressing GFP (S6D and S6E Fig). As a more sensitive assay, we used mass spectrometry to measure levels of the major PE and PC species in fat body [38–40]. In agreement with our TLC results, we found increased total levels of PE and PC in fat bodies expressing Toll^10b compared with controls (Fig 4H). We note that our TLC and mass spectrometry data show higher levels of PE than PC in both genotypes, as is true of membrane phospholipid composition in flies, and opposite to the pattern in mammals, where PC predominates [41]. Levels of most major PE and PC species were increased 1.5- to 2-fold in Toll^10b-expressing fat bodies compared with controls (Fig 4I). These data suggest that increased expression of Kennedy pathway enzymes translates to substantial changes in phospholipid metabolism in response to immune signaling.

Transcriptional control of Kennedy pathway enzymes in cells with active Toll signaling

We asked whether the canonical downstream transcription factor in the Toll signaling pathway, the NF-κB family member Dif, was sufficient to regulate expression of Kennedy pathway enzymes. Elevated expression of Dif in fat body using the r4-GAL4 driver induced expression of both eas and Pcyt1 (S7A Fig). Dif also phenocopied Toll^10b in the regulation of the lipogenic genes Lipin and midway, leading to reductions in transcript levels for both of these genes (S7B Fig). These lipid metabolic enzymes are not known to be direct transcriptional targets of Dif, so we asked whether other transcription factors might act downstream of Toll and Dif to regulate lipid metabolism.

We investigated the role of two transcription factors known to participate in phospholipid homeostasis–Sterol regulatory element binding protein (SREBP) and X-box binding protein-1 (Xbp1)–in the elevated expression of Kennedy pathway enzymes in fat bodies with active Toll signaling. In flies, as in mammals, the transcription factor SREBP is a key regulator of de novo lipogenesis [42]. We find that fat bodies expressing Toll^10b exhibit elevated expression of SREBP mRNA and protein (S8A and S8B Fig). However, fat body-specific knockdown of SREBP did not affect either basal or Toll signaling-induced expression of eas, CG7149, or CG2201. Although basal expression of Pcyt1 was reduced nearly three-fold by loss of SREBP, activation of Toll signaling led to 1.8-fold induction of Pcyt1 in fat bodies regardless of SREBP levels (S8C Fig).

The unfolded protein response mediator Xbp1 regulates de novo lipogenesis of membrane phospholipids to permit ER expansion [43]. Xbp1 is activated by Ire1-mediated splicing [44,45], and fat bodies expressing Toll^10b under control of r4-GAL4 (Fig 5A) or cg-GAL4 (S3G Fig) exhibit elevated levels of spliced Xbp1 compared with controls. Levels of unspliced Xbp1 were largely unchanged by expression of Toll^10b in fat body (Fig 5A). Forced expression of Dif in fat body also induced Xbp1 splicing, and loss of Dif in fat bodies expressing Toll^10b prevented the increase in spliced Xbp1 (S7C Fig). In larvae subjected to sterile injury or infection with Enterococcus faecalis, levels of spliced Xbp1 were increased between 1.3- to 1.9-fold compared with uninfected controls at 6, 18, 24, and 36 hours following infection (Fig 5B). To test the role of Xbp1 in regulation of Kennedy pathway enzymes by innate immune signaling, we attempted Xbp1 loss-of-function experiments in fat bodies co-expressing Toll^10b. Xbp1 null mutants die as second instar larvae [46], and chronic knockdown of Xbp1 in larval fat body via r4-GAL4 strongly inhibits whole-animal growth. However, acute induction of both Toll^10b and Xbp1^RNAi transgenes in fat body using temperature-sensitive TubGAL80ts to restrict r4-GAL4 activity to a 24-hour period led to a near-complete loss of Xbp1 transcripts (Fig 5A). Knocking down Xbp1 blunted Toll^10b-dependent induction of eas and blocked Toll^10b-dependent induction of CG7149 (Fig 5C). Acute loss of Xbp1 in fat body reduced basal levels of Pcyt1 transcripts. Acute expression of Toll^10b induced Pcyt1 1.6-fold compared with controls, but only 1.2-fold in fat bodies with simultaneous loss of Xbp1 (Fig 5D). We did not observe induction of CG2201 in this experiment, perhaps due to the acute nature of Toll^10b expression (Fig 5D). Finally, acute loss of Xbp1 blunted induction of SREBP and the AMP Drs by Toll signaling (S8E Fig); we did not observe changes in induction of Drs or Xbp1 splicing when SREBP was manipulated (S8D Fig).

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Fig 5. Toll signaling induces Kennedy pathway enzymes in a Xbp1-dependent manner.

(A) Transcript levels of unspliced (left) and spliced (right) Xbp1 were measured by RT-qPCR in late third instar larval fat bodies with GAL80ts-mediated induction of Toll^10b with or without Xbp1^RNAi for 24 hours at 30°C, n = 6/group. ***p = 0.0001 and ****p < 0.0001 versus fat bodies acutely expressing RFP+GFP. (B) Transcript levels of spliced Xbp1, normalized to Rp49, in third instar larval fat bodies from uninfected controls, larvae subjected to sterile injury, and larvae infected with Enterococcus faecalis at 6–36 hours post infection n = 7-10/group. *p ≤ 0.0484, **p ≤ 0.0081, and ****p < 0.0001 versus uninfected controls. (C, D) Transcript levels of indicated genes in late third instar larval fat bodies with GAL80ts-mediated induction of Toll^10b with or without Xbp1^RNAi for 24 hours at 30°C, n = 6/group. **p = 0.0087, ***p = 0.0001, and ****p < 0.0001 versus fat bodies acutely expressing RFP+GFP. Data are presented as means ± SD. p values were determined by one-way ANOVA with Dunnett’s (A, B) or the Tukey-Kramer multiple comparisons test (C, D).

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In addition to the Ire1/Xbp1 pathway, the unfolded protein response is initiated by two other stress sensors: protein kinase R-like ER kinase (PERK, also called EIF2AK3, and named PEK in Drosophila) and activating transcription factor 6 (Atf6) [47]. We asked whether these other arms of the unfolded protein response played a role in the induction of the Kennedy pathway by Toll signaling. Toll signaling in fat body leads to a significant reduction in fat body PEK mRNA levels, and we find that loss of PEK does not lead to significant changes in transcript levels of Drs, eas, or Pcyt1 in animals with active Toll signaling in fat body (S9A Fig). In contrast, Atf6 expression is modestly increased in response to fat body Toll signaling (S9B Fig). While Toll^10b induces a 2.5-fold increase in eas compared with control fat bodies, loss of Atf6 blunts this effect of Toll, leading to only a 1.5-fold increase in eas expression relative to fat bodies with Atf6 knockdown alone. Effects of Atf6 on Pcyt1 expression were difficult to interpret due to variance in expression of this enzyme in fat bodies expressing Atf6^RNAi (S9B Fig). Together, these results suggest that Toll signaling in the fat body induces two arms of the unfolded protein response: Xbp1, downstream of Ire1, and Atf6, and that Xbp1 participates in both basal and stimulated expression of Kennedy pathway enzymes.

Toll signaling leads to ER expansion

The induction of Xbp1 splicing suggested that the ER unfolded protein response might be induced in fat bodies with active innate immune signaling. We therefore examined expression of canonical Xbp1 targets that participate in the unfolded protein response [48,49]. We found significantly elevated expression of binding immunoglobulin protein (BiP, encoded by Hsc70-3), protein disulfide isomerase (Pdi), ER degradation enhancer mannosidase alpha-like 1 (Edem1), and Sec24cd in response to chronic Toll signaling (Figs 6A and S3G).

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Fig 6. Chronic fat body Toll signaling leads to expansion of the endoplasmic reticulum.

(A) Late third instar fat body levels of Hsc70-3 (BiP), Pdi, Edem1, and Sec24cd were measured by RT-qPCR and normalized to Rp49, n = 4-8/group. **p = 0.0012, ***p = 0.001, and ****p < 0.0001 versus GFP. (B) Electron micrographs of fat body cells expressing GFP (left) or Toll^10b (right) under control of r4-GAL4. ER, endoplasmic reticulum; LD, lipid droplet; g, glycogen; mt, mitochondria. Dashed blue lines show areas enlarged in (C and C’). Scale bar, 1 μm. (C) Insets of electron micrographs outlined in blue dashed boxes in (B). (C’) Insets with ER structures highlighted in purple. Scale bar, 1μm. (D) Stereological analysis of organelle- and feature-specific volume density. Data points represent the average of volume densities measured from 5–15 images from a single fat body, n = 4–5 fat bodies/group. *p = 0.0175 and **p ≤ 0.0044 versus GFP. Data are presented as means ± SD. p values were determined by Student’s t test (A,D).

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We next used transmission electron microscopy to examine whether ER morphology is altered by innate immune signaling. We observed dilated ER in fat body cells with active Toll signaling compared with controls (Fig 6B–6C’). To quantify this difference, we used stereological tools to compare organelles and features such as glycogen and cytosol between genotypes. This analysis revealed a 40% increase in the relative volume of ER with a simultaneous 40% decrease in the relative volume of organelle-free cytosol in response to Toll pathway activation. We find no significant difference in the relative volume of lipid droplets, but we observe an increase in glycogen volume in fat body cells expressing Toll^10b compared with controls, in agreement with our finding of increased glycogen levels in the larval fat body (Fig 6D and refer back to Fig 2B).

AMP synthesis contributes to induction of Kennedy pathway enzymes

In response to infection with fungi or Gram-positive bacteria, the Toll signaling pathway drives synthesis and secretion of large quantities of AMPs via the classical ER-Golgi-secretory vesicle pathway [50]. Individual AMPs have been measured at levels close to 100 μM in hemolymph, which represents secretion of millions of individual proteins [16]. Acute activation of Toll signaling leads to 4- to 410-fold induction of 17 of the 37 AMPs encoded in the Drosophila genome (S10A Fig) [26]. We reasoned that ER expansion and phospholipid synthesis may serve the process of AMP production and secretion. To test this hypothesis, we asked whether induction of Kennedy pathway enzymes occurs downstream of AMP synthesis. The Bom^Δ55C mutation deletes a cluster of ten highly-induced genes, the Bomanins, that are critical for survival during infection [51], and the Drs^Δ7–17 mutation is a deletion of Drosomycin [28]. Together, the genes encoded in the Bom^55C cluster and Drs account for 80% of the AMP transcripts that are induced by Toll signaling (Fig 7A). In a series of genetic manipulations to curtail AMP production, we drove Toll^10b in fat bodies of larvae carrying Bom^Δ55C and Drs^Δ7–17 mutations in single or double homozygous combinations and measured expression of the Kennedy pathway enzymes eas and Pcyt1. The Bom^Δ55C and Drs^Δ7–17 mutations, alone or in combination, led to the expected decreases in Toll^10b-induced expression of the Bom^55C gene BomS2 and Drs (Fig 7B). Unmanipulated AMPs such as Daisho1 and Daisho2 (also known as IM4 and IM14) [52] were still induced by Toll^10b in Bom^Δ55C and Drs^Δ7–17 single or double mutants (S10B Fig). In larval fat bodies expressing GFP, mutation of Bom^55C and Drs did not alter low, basal levels of eas or Pcyt1. However, Bom^Δ55C; Drs^Δ7–17 double homozygotes expressing Toll^10b exhibited only a 1.6-fold induction of eas and a 1.3-fold induction of Pcyt1. In contrast, in larvae with a full complement of AMPs, Toll signaling elicited a 4.1-fold induction of eas and a 2.1-fold induction of Pcyt1 (Fig 7C and 7D). These data suggest that induction of Kennedy pathway enzymes in response to Toll signaling is closely linked to the level of AMP synthesis.

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Fig 7. Induction of antimicrobial peptide synthesis contributes to regulation of eas and Pcyt1 by Toll signaling.

(A) Toll signaling induces expression of 17 AMPs. AMPs clustered on chromosome 2 (deleted in the Bom^Δ55C mutant) represent 70% of the AMP transcripts induced by Toll^10b expression. Drs, deleted in the Drs^Δ7–17 mutant, represents 10% of the induced AMP transcripts. (B) Transcript levels of BomS2 (left) and Drs (right), normalized to Rp49, in fat bodies of late third instar larvae expressing GFP (closed symbols) or Toll^10b (open symbols) under r4-GAL4 control. Animals were wild type, heterozygous, or homozygous for Drs^Δ7–17 and Bom^Δ55C as indicated, n = 5-8/group. *p = 0.0145, **p = 0.0015, and ****p < 0.0001 versus GFP-expressing controls with the same Drs^Δ7–17 and Bom^Δ55C genotypes. Note that GFP-expressing controls are heterozygous for Drs^Δ7–17 while Toll^10b-expressing larvae are homozygous for Drs^Δ7–17. (C, D) Transcript levels of eas (C) and Pcyt1 (D), normalized to Rp49, in fat bodies of late third instar larvae expressing GFP (closed symbols) or Toll^10b (open symbols) under r4-GAL4 control. Animals were wild type, heterozygous, or homozygous for Drs^Δ7–17 and Bom^Δ55C as indicated, n = 5-8/group (blue open and closed symbols) and n = 11-14/group for animals expressing GFP or Toll^10b in fat body on a wild type background (black open and closed symbols). *p ≤ 0.0409, **p ≤ 0.032, ***p = 0.0009, and ****p < 0.0001. (E, F) Transcript levels of spliced Xbp1 (E) and Hsc70-3 (BiP) (F), normalized to Rp49, in fat bodies of late third instar larvae expressing GFP (closed symbols) or Toll^10b (open symbols) under r4-GAL4 control. Animals were wild type, heterozygous, or homozygous for Drs^Δ7–17 and Bom^Δ55C as indicated, n = 5-8/group (blue open and closed symbols), n = 11-13/group (spliced Xbp1), and n = 4-6/group (Hsc70-3 (BiP)) for animals expressing GFP or Toll^10b in fat body on a wild type background (black open and closed symbols). **p = 0.0086, ***p = 0.0004, and ****p < 0.0001. Data are presented as means ± SD. p values were determined by Student’s t test.

https://doi.org/10.1371/journal.pgen.1009192.g007

Next, we asked whether ER stress genes were also suppressed in Bom^Δ55C; Drs^Δ7–17 double homozygous fat bodies. Surprisingly, transcript levels of spliced Xbp1 and Hsc70-3 (BiP) were increased in these fat bodies compared with controls and were elevated above levels observed in animals expressing Toll^10b with a full complement of AMP genes (Fig 7E and 7F). These data challenged our previous findings that Xbp1 positively contributes to regulation of Pcyt1 and eas downstream of Toll receptor activation. This result led us to consider why ER stress might be induced in cells lacking a substantial portion of the AMP genes that would contribute to the secretory burden induced by Toll.

We surmised that fat bodies undergoing immune signaling but with impaired induction of Pcyt1 and eas might exhibit a relative lack of capacity for phospholipid synthesis. Because changes in phospholipid synthesis can induce ER stress [53,54], we measured expression of ER stress genes in fat bodies with simultaneous knockdown of Pcyt1 and eas. Expression of RNAi transgenes targeting these enzymes strongly reduced their levels in fat bodies (S11A Fig). As we saw previously, expression of Toll^10b in fat body led to increased protein levels of both Pcyt1 and eas, and co-expression of Toll^10b with RNAi transgenes targeting these enzymes strongly reduced Pcyt1 expression and normalized eas to control levels (S11A Fig). We note that reduced Pcyt1 and eas expression in fat body did not restore triglyceride levels in animals co-expressing Toll^10b in fat body (S11B Fig). We measured transcript levels of spliced Xbp1 and its targets Hsc70-3 (BiP), Pdi, and Sec24cd in fat bodies expressing control transgenes or Pcyt1^RNAi and eas^RNAi with or without co-expression of Toll^l0b. We find that, indeed, loss of these Kennedy pathway enzymes induces Xbp1 splicing and expression of Hsc70-3 (BiP) and Sec24cd in control fat bodies and those expressing Toll^10b (S11C Fig).

Pcyt1 and easily shocked support the innate immune response

To determine whether Pcyt1 and eas play a functional role in the immune response, we assessed two immune functions, AMP production and bacterial clearance, in larvae with simultaneous knockdown of Pcyt1 and eas in fat body. First, we genetically activated the Toll signaling pathway by expressing Toll^10b in larval fat bodies. To evaluate AMP secretion, we used a DrsGFP transgene that is a fusion protein of Drosomycin and GFP under the control of 2.5 kb of the Drosomycin promoter upstream of the start codon. This DrsGFP construct retains the signal peptide necessary for AMP secretion into hemolymph. This allowed us to assess secretion of Drosomycin from the fat body into hemolymph during active Toll signaling. In male flies expressing Toll^10b in fat body, we observed increased protein levels of DrsGFP, Pcyt1, and eas in the fat body. Knockdown of Pcyt1 and eas in fat body led to a strong, tissue-autonomous decrease in DrsGFP protein levels (Fig 8A). DrsGFP is undetectable in hemolymph from control larvae expressing RFP in fat body. Secretion of DrsGFP into hemolymph is markedly increased by fat body Toll signaling, but this is reduced when Pcyt1 and eas are knocked down (Fig 8A). In female larvae, knockdown of Pcyt1 and eas in fat bodies with active Toll signaling did not have an appreciable effect on DrsGFP expression in fat body or secretion into hemolymph, raising the possibility that the humoral immune response operates differently in males and females (Fig 8B).

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Fig 8. Pcyt1 and easily shocked support the innate immune response.

(A, B) Western blot analysis of DrsGFP, Pcyt1, and eas protein levels in fat bodies (top four panels) and DrsGFP in hemolymph (bottom panel) from male (A) and female (B) late third instar larvae expressing RFP or Toll^10b alone or with simultaneous knockdown of Pcyt1 and easily shocked using r4-GAL4. Histone H3 levels are shown as loading controls for fat body blots. For hemolymph blots, equivalent volumes of diluted hemolymph (equal to 50 nL of neat hemolymph) were loaded. (C-F) Top: Whole-animal transcript levels of Drs, and bottom: transcript levels of Enterococcus faecalis 16S rRNA in larvae expressing RFP+GFP or Pcyt1^RNAi+eas^RNAi in fat body under control of r4-GAL4 at 6–36 hours post infection with Enterococcus faecalis. Both Drs and Enterococcus faecalis 16S rRNA were normalized to Rp49. Uninfected control samples for each genotype were collected throughout the time course, pooled, and are plotted on each graph. All groups were normalized to the pooled, uninfected RFP+GFP samples, n = 6-15/group. *p ≤ 0.0310, **p ≤ 0.0097, ***p ≤ 0.0007, and ****p < 0.0001 versus infected RFP+GFP or versus uninfected controls of the same genotype, as indicated. Data are presented as means ± SD. p values were determined by one-way ANOVA with the Tukey-Kramer multiple comparisons test (C-F).

https://doi.org/10.1371/journal.pgen.1009192.g008

Finally, we investigated the role of Pcyt1 and eas in the ability of the animal to elicit an appropriate immune response via induction of Drosomycin and to properly clear the bacterial insult. We infected larvae with Enterococcus faecalis and measured Drs mRNA and E. faecalis 16S rRNA in the same samples of total RNA isolated from whole larvae at various time points following infection. By 6 hours post infection, animals expressing RFP and GFP control transgenes in fat body and animals with simultaneous knockdown of Pcyt1 and eas have induced Drs gene expression, although Drs mRNA levels in the knockdown animals were significantly lower compared with RFP and GFP controls. At this time point we observed 16-54-fold higher levels of E. faecalis 16S rRNA in infected animals compared with uninfected controls (Fig 8C). We note that E. faecalis is a minor component of the Drosophila microbiome [55], and low levels of 16S rRNA were detected in controls of both genotypes. By 12 hours post infection, the knockdown animals succeeded in inducing Drs transcripts and matched controls in the amount of E. faecalis bacterial transcripts (Fig 8D). Thus, at early time points of infection, knockdown animals lag in their ability to induce Drs compared with control animals. At 24 hours post infection, infected animals with fat body knockdown of Pcyt1 and eas exhibited significantly increased Drs levels compared with infected controls. This difference correlates with 22-fold higher mean levels of bacterial 16S rRNA in the infected knockdown animals compared with infected controls (Fig 8E). By 36 hours post infection, Drs levels in control animals expressing RFP and GFP in fat body were equivalent in uninfected and infected groups. In contrast, infected animals with loss of Pcyt1 and eas in fat body sustained an increase in Drs levels (Fig 8F). Mean values of E. faecalis 16S rRNA were 56-fold higher in infected knockdown animals compared with infected controls expressing RFP and GFP, although this difference is not statistically significant (Fig 8F). We note that Drs levels at 24 and 36 hours post infection correlate with bacterial load, suggesting that, at these late time points, Drs expression is driven by persistence of bacteria in the animal, rather than from the initial stress of injury. These data suggest that the metabolic switch to phospholipid synthesis in the fat body in response to immune signaling may contribute to the ability of the animal to control the microbial burden during an infection.

Drosophila stocks and husbandry

Flies were raised on food containing 7.8% molasses, 2.4% yeast, 4.6% cornmeal, 0.3% propionic acid, and 0.1% methylparaben (Archon Scientific, Durham, NC). Except where noted, experiments were performed using mid- to late-third instar larvae (96–108 h after egg lay). The following stocks were obtained from the Bloomington Drosophila Stock Center (Bloomington, IN): UAS-RFP (2nd (#30556) and 3rd (#31417) chromosome insertions), UAS-GFP (2nd (#1521) and 3rd (#1522) chromosome insertions), UAS-Dif^RNAi (#30513), mdy^QX25 (#5095), mdy^EY07280 (#20167), UAS-midway^RNAi (#65963), UAS-Lipin^RNAi (#63614), UAS-Pcyt1^RNAi (#62156), UAS-Xbp1^RNAi (#36755), UAS-Atf6^RNAi (#26211), UAS-PEK^RNAi (#42499), DiptLacZ, DrsGFP (#55707), r4-GAL4 (#33832) and cg-GAL4 (#7011). UAS-SREBP^RNAi (#37640) and UAS-eas^RNAi (#34287) were obtained from the Vienna Drosophila Resource Center. Other flies used were: UAS-Toll^10b [75], UAS-Dif [76], DrsGFP [30], UAS-Lipin [36], Bom^Δ55C [51], and Drs^Δ7–17 [28]. Full genotypes of flies are listed in S1 Table.

Construction of UAS-HA.mdy transgenic flies

The full-length midway (mdy) cDNA was amplified by PCR from clone LD33582 (Drosophila Genomics Resource Center, Bloomington, IN) using gene-specific primers engineered to contain an amino-terminal HA tag (see S2 Table). PCR products were cloned into pENTR (Invitrogen) and validated by sequencing. Gateway cloning (Invitrogen) was used to generate pUAST-HA.mdy. This construct was injected into Drosophila embryos at Rainbow Transgenics (Camarillo, CA). Standard genetics was used to map and generate balanced transgenic lines.

Bacterial infection

Early third instar larvae (~72–78 h after egg lay) were removed from bottles, rinsed in PBS, sorted for fluorescent transgene expression as indicated, and transferred to fresh food. For bacterial infections, larvae were transferred briefly to a pool of the Gram-positive bacteria Enterococcus faecalis (strain OG1RF, ATCC 47077), grown in brain heart infusion media with rifampicin, and concentrated to OD₆₀₀ 10.0 in PBS. Larvae coated in E. faecalis were then transferred to a 50 μL pool of mineral oil (M5904, Sigma-Aldrich) on a stack of eight parafilm sheets. Punctures were performed in mineral oil, which provided sufficient hydrostatic pressure to prevent the gut and fat body from bursting out of the wound site, as regularly occurs when punctures are performed in PBS. For bacterial infections and sterile injury, larvae were punctured laterally in abdominal segment 7 or 8 with a tungsten carbide needle with a one μm tip (10130–10, Fine Science Tools) [26,77]. Larvae in the sterile injury groups were transferred directly to mineral oil and punctured, and control larvae were transferred directly to mineral oil. Larvae in all three groups were kept in mineral oil on ice for ten minutes before transfer to fresh food for indicated times. Uninfected controls and sterile injury or bacteria-infected larvae bearing melanotic clots at puncture sites were collected individually for measurement of triglyceride levels and DrsGFP expression. Fat bodies dissected from two animals per sample or single, whole larvae were used for RNA isolation.

Triglyceride and protein measurements

Whole larvae or dissected organs were flash frozen on dry ice. Samples were sonicated three times for 10 seconds each time in 140 mM NaCl, 50 mM Tris-HCl, pH 7.4, 0.1% Triton X-100 with protease inhibitors (Roche). Following clearing by centrifugation at 4°C, supernatants were transferred to new tubes. Triglyceride (Liquicolor Test, Stanbio) and protein (BCA assay, Pierce) were measured in each sample, and triglyceride levels were normalized to protein levels.

Hemolymph trehalose and glucose

Hemolymph was collected on ice from mid-third instar larvae (hemolymph from 8–10 larvae pooled per sample). Endogenous trehalase was destroyed by heating hemolymph diluted in PBS at 70°C for 20 min. The sample was split in half, 1 mU trehalase (Sigma-Aldrich, T8778) was added to one tube, and both were incubated at 37°C for 2 h. Glucose was measured in both samples (GAGO20 kit, Sigma-Aldrich). Trehalose was calculated by subtracting glucose values in trehalase-free samples from glucose values in trehalase-treated samples, and then dividing by two as trehalose is a dimer of glucose. Trehalose and glucose values were normalized to hemolymph volume.

Glycogen and free glucose measurements

Whole larvae or dissected organs were flash frozen on dry ice. Samples were homogenized using a Kontes pestle in 0.1 M NaOH. Following clearing by centrifugation at 4°C, samples were incubated for 1h at 37°C with 0.2 M NaOAc, pH 4.8 with or without 5 mg/mL amyloglucosidase (Sigma-Aldrich, A7420). Samples were then incubated for 10 min at room temperature with assay buffer including glucose oxidase (0.25 U/mL, Sigma-Aldrich, G7141), horseradish peroxidase (0.17 U/mL, Sigma-Aldrich, P8250), and Amplex Red (20 μM, Invitrogen, A36006). Fluorescence was measured (excitation/emission maxima = 535/587 nm) using an Infinite 200 PRO plate reader (Tecan). Glycogen was calculated as the amount of amyloglucosidase-hydrolyzed glucose, and free glucose is the amount of glucose measured in the sample without hydrolysis via amyloglucosidase. Glycogen and free glucose levels were normalized first to glucose standards (Fisher CAS 50-99-7) and then to protein levels (BCA assay, Pierce).

Starvation

Two day-old adult male and female flies that expressed GFP or Toll^10b throughout the larval stage under control of r4-GAL4 were transferred to empty plastic vials (n = 9-11/vial) that were capped with a foam stopper. For starvation with water, the foam stopper was regularly saturated with water, such that water for consumption was available on the bottom side of the stopper at all times. Throughout the day, the number of dead flies was recorded every three hours.

Western blot analysis and antibodies

Fat bodies (n = 4–6 pooled/sample) were sonicated in lysis buffer (2% SDS, 60 mM Tris-HCl, pH 6.8) with phosphatase and protease inhibitors (Roche). For hemolymph blots, hemolymph was collected from five larvae and pooled, and dilute hemolymph (equivalent to 50 nL of neat hemolymph) was separated by SDS-PAGE. Equal amounts of fat body or whole larval protein (10–40 μg/lane, measured using a BCA assay (Pierce)) were separated by SDS-PAGE, transferred to nitrocellulose, blocked in 3% milk in 1X TBS with 0.2% Tween 20 (TBS-T), and blotted overnight at 4°C with primary antibodies diluted in 1% milk in TBS-T. Following multiple washes in TBS-T, secondary antibodies were incubated in 1% milk in TBS-T for 2h at room temperature, washed again, incubated with ECL (Pierce), and exposed to film. Antibodies used were: rabbit anti-GFP (A11122, Invitrogen), rabbit anti-human Histone H3 and rabbit anti-HA (4499 and 3724, Cell Signaling Technology), rabbit anti-Drosophila easily shocked [78], guinea pig anti-Drosophila Pcyt1 [35], mouse anti-human SREBP1 (SC-13551, Santa Cruz Biotechnology), rabbit anti-GFP (A11122, Invitrogen), goat anti-rabbit HRP and goat anti-mouse HRP (111-035-003 and 115-035-003, Jackson ImmunoResearch), and goat anti-guinea pig HRP (6090–05, SouthernBiotech).

Quantitative RT-PCR

Total RNA was extracted from whole larvae or mid to late third instar fat bodies (n = 2–6 pooled/sample) using a Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 μg) was used to generate cDNA using a High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific). Gene expression was measured using gene-specific primers. Quantitative PCR reactions were performed on 10–20 ng cDNA using SYBR Select Master Mix (Thermo Fisher Scientific) with a Bio-Rad CFX Connect Real-Time PCR Detection System. Relative amounts of transcripts were calculated using the comparative Ct method with Rp49 as a reference gene [79]. Gene-specific primer sequences are listed in S2 Table.

Lipin activity

Lipin activity was measured using Triton X-100 micelles as previously reported [80]. Briefly, radiolabeled [³²P]PA substrate was prepared by phosphorylating 1,2-Dioleoyl-sn-glycerol (800811, Avanti Polar Lipids) with E. coli diacylglycerol kinase (D3065, Sigma-Aldrich) and [γ-³²P]ATP (NEG035C005MC, Perkin Elmer) and purified by thin-layer chromatography [81]. To prepare the micelles, Triton X-100 was mixed with buffer A (50 mM Tris-HCl, 10 mM 2-mercaptoethanol, pH 7.4) to a final concentration of 10 mM. Next, 1 μmol of unlabeled 1,2-Dioleoyl-sn-glycero-3-phosphate (840875, Avanti Polar Lipids) was dissolved in chloroform and mixed with [³²P]PA (3,000 cpm/nmol) in a glass tube, dried to a thin film under N₂ gas, and resuspended with 1 mL of 10 mM Triton X-100. The measurement of PAP activity was determined by following the release of the radiolabeled phosphate from [³²P]PA. Lysates prepared from larval fat bodies, radioactive micelles, and buffer A were combined to a final volume of 100 μL. The final concentrations for all reactions were as follows: 50 mM Tris-HCl, 10 mM 2-mercaptoethanol, and 0.2 mM PA. Total PAP activity was measured by including 0.5 mM MgCl₂. PAP activity for Mg²⁺-independent enzymes was measured by instead including 1 mM EDTA. The reactions were allowed to proceed for 20 min at 30°C with gentle agitation and were terminated with the addition of 500 μL of acidified methanol (MeOH·0.1 N HCl). Free phosphate was extracted with the addition of 1 mL chloroform followed by 1 mL 1 M MgCl₂. The organic extraction was vortexed and 500 μL of the aqueous phase was transferred to a scintillation vial to measure the removal of ³²P from PA using a scintillation counter. The activity from assays containing lysate was normalized to activity in assays without enzyme present. PAP activity specific to Lipin was calculated by subtracting the Mg²⁺-independent activity from the total PAP activity and normalizing to total protein levels in each lysate.

Thin layer chromatography

Late third instar larval fat bodies (n = 8 pooled/sample) were sonicated in 1X TBS. Fat body lysates underwent lipid extraction using a modified Bligh-Dyer method (chloroform: methanol: 0.2 M NaCl, ratio 2:2:1) [82]. Organic extracts were dried and resuspended in 30 μL of chloroform: methanol (1:1) for quantitation by thin layer chromatography. Using a Hamilton syringe (#701), 15–20 μL of resuspended extracts were spotted on the bottoms of silica gel plates (Millipore HPTLC Silica Gel Glass plates 105631) at starting points drawn in pencil. Standards were phosphatidylcholine (3 μL at 1 μg/μL; egg L-α-phosphatidylcholine Avanti Polar Lipids, 131601) and phosphatidylethanolamine (3 μL at 1 μg/μL; egg L-α-phosphatidylethanolamine Avanti Polar Lipids, 840021). During loading, samples were spotted beneath an argon gas flow to allow for rapid drying and to minimize oxidation. Silica gel plates were developed in a glass TLC tank in chloroform: methanol: acetic acid: water in a ratio of 75:35:6:2, removed, air dried, and stained overnight with iodine vapor in a separate glass TLC tank maintained in a fume hood. Following staining, plates were immediately scanned, and images were used for densitometry analysis in ImageJ to measure the area of each PE and PC spot, recognized based on the location and migration pattern of the corresponding standard. Areas of lipid spots were normalized to total protein in lysates (measured by BCA assay, Pierce) and volumes of lysates used in the initial organic extraction.

Liquid chromatography and mass spectrometry

Larval fat bodies (n = 6 pooled/sample) were sonicated in ultrapure water and protein was measured (BCA assay, Pierce). Fat body lysates (normalized to 40 μg protein) underwent lipid extraction using a modified Bligh-Dyer method (chloroform: methanol: ultrapure water, ratio 2:2:1), adding 5 μg 1,2-dinonadecanoyl-sn-glycero-3-phosphocholine (DNPC, Avanti Polar Lipids, 850320) to control for extraction efficiency. Organic extracts were dried and resuspended in chloroform: methanol (1:1) for quantification by liquid chromatography-coupled mass spectrometry. Lipids were separated on an EVO C18 column (Kinetex 5 μm, 100 x 4.6 mm, Phenomenex), using a binary gradient consisting of Solvent A (69% methanol, 31% water with 10 mM ammonium acetate) and Solvent B (50% methanol, 50% isopropanol with 10 mM ammonium acetate) as the mobile phases. Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species identification and relative quantitation was achieved by liquid chromatography-linked electrospray ionization mass spectrometry on a 4000 QTRAP triple-quadrupole mass spectrometer (AB Sciex). Identification of phospholipids of interest, corresponding to PC and PE species with acyl chain lengths previously described to be abundant in larval fat body [38–40], was performed in positive ion mode via multiple reaction monitoring [83].

Electron Microscopy

Larval fat bodies were dissected and fixed initially in 2% glutaraldehyde, 2.5% formaldehyde, 0.1 M Na cacodylate, pH 7.4 (30 min at room temperature followed by 1h on ice). They were then post-fixed in 1% OsO₄, 0.1 M Na cacodylate, pH 7.4 (15 min on ice and 45 min at room temperature), washed three times for 5 min in 0.1 M Na acetate, pH 6.0, and then stained in-block overnight at room temperature in 0.5% uranyl acetate in the same buffer. The samples were then dehydrated in acetone (70, 95, and 100%), exchanged from acetone into Epon resin, and embedded in fresh Epon. 70 nm sections from embedded fat bodies were mounted on copper grids, stained sequentially with uranyl acetate and lead citrate, and examined by transmission electron microscopy.

Stereology

All images for stereology were taken in an unbiased manner at 6000X. Three different blocks of each genotype (r4>GFP or r4>Toll^10b) that each contained fat bodies from three to four larvae were used for quantitative analysis. Biological replicates refer to sets of adjacent sections cut from separate blocks or from different depths of the same block to expose different fat body tissue. STEPanizer software (Java) was used for stereology analysis [84]. In order to measure relative volumes of features within sections, images were analyzed using nine-line tile pairs per image [85]. Point counts were made to determine relative volume of organelles comprising the entire cytoplasmic landscape in each image. Batch mode was used to analyze images from each block for each genotype.

Quantitation and statistical analysis

Statistical parameters including exact sample sizes (with n referring to biological replicates), data plotted (typically means ± SD), exact p values, and statistical tests used are reported in Figure Legends. Statistical analyses were performed using Graphpad Prism 8. Survival of starved flies was analyzed by the log-rank test. All other data were analyzed by Student’s t test or by one-way ANOVA with the Tukey-Kramer or Dunnett’s multiple comparisons test.