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Open Access

Correction

Correction: OVATE Family Protein 8 Positively Mediates Brassinosteroid Signaling through Interacting with the GSK3-like Kinase in Rice

  • Chao Yang,
  • Wenjin Shen,
  • Yong He,
  • Zhihong Tian,
  • Jianxiong Li

There is an error in panel A of Fig 3. Specifically, the yeast-two-hybrid (Y2H) results are duplicated for AD-DLT+BD-OsOFP8 and AD-OsBZR1+BD-OsOFP8 on the SD-Leu-Trp plate, and the positive control is missing from this panel. Please see the correct version of Fig 3 and its associated caption below.

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Fig 3. OsGSK2 interacts with and phosphorylates OsOFP8.

(A) Yeast two-hybrid analysis for the interaction between OsOFP8 and OsGSK2,DLT, and OsBZR1. Co-transformed yeast clones were placed on SD dropout plates to detect the interactions. SD-Leu-Trp: synthetic complete medium lacking Leu and Trp for co-transformation detection. SD-Leu-Trp-His-Ade: synthetic complete medium lacking Trp, Leu, His, and Ade for interaction detection. Positive control, pGADT7-T (SV40 large T antigen) / pGBKT7-53 (murine p53). (B) Immunoprecipitation (IP) assay shows that OsOFP8 is associated with OsGSK2. Arabidopsis protoplasts expressing either YFP and 3HA-OsGSK2 or OsOFP8-YFP and 3HA-OsGSK2 were subjected to protein extraction. The Input (cell lysates) and IP were immunoblotted with indicated antibodies. WB:GFP indicates western blotting with GFP antibody. WB:HA indicates western blotting with HA antibody. (C) BiFC assay shows the interaction between OsOFP8 and OsGSK2. Chl means chlorophyll. (D) OsOFP8 phosphorylation analysis. Arabidopsis protoplast cells expressing OsOFP8-YFP only or OsOFP8-YFP with 3HA-OsGSK2 were subjected to protein extraction and then immunoprecipitated with either GFP antibody (WB: anti-GFP) or HA antibody (WB: anti-HA). Phosphorylation was detected with biotin-pendant Zn2+-Phos-tag (BTL-111). Black and white arrowheads indicate OsOFP8-YFP and 3HA-OsGSK2, respectively. Black and white arrows indicate phosphorylated OsOFP8-YFP-P and 3HA-OsGSK2-P, respectively. * represents the internally phosphorylated OsOFP8-YFP.

https://doi.org/10.1371/journal.pgen.1006970.g001

Reference

  1. 1. Yang C, Shen W, He Y, Tian Z, Li J (2016) OVATE Family Protein 8 Positively Mediates Brassinosteroid Signaling through Interacting with the GSK3-like Kinase in Rice. PLoS Genet 12(6): e1006118. pmid:27332964

Citation: Yang C, Shen W, He Y, Tian Z, Li J (2017) Correction: OVATE Family Protein 8 Positively Mediates Brassinosteroid Signaling through Interacting with the GSK3-like Kinase in Rice. PLoS Genet 13(8): e1006970. https://doi.org/10.1371/journal.pgen.1006970

Published: August 22, 2017

Copyright: © 2017 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.