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PLoS Genetics Issue Image | Vol. 22(7) August 2026

Unintended HDR Template Integration Rivals Desired Genome Edits in Drosophila

Composite image of a Drosophila melanogaster head illustrating the outcome of genome editing followed by HDR repair. Red fluorescent eyed Drosophila carry the desired genome edit; green fluorescent eyed Drosophila harbor undesired template vector genome insertional events. The desired and undesired outcomes occur at almost equal frequency, underscoring the need for systematic screening against template vector integration following genome editing. Källstig et al. 2026

Image Credit: Evelyne Ruchti and Brian McCabe

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Unintended HDR Template Integration Rivals Desired Genome Edits in Drosophila

Composite image of a Drosophila melanogaster head illustrating the outcome of genome editing followed by HDR repair. Red fluorescent eyed Drosophila carry the desired genome edit; green fluorescent eyed Drosophila harbor undesired template vector genome insertional events. The desired and undesired outcomes occur at almost equal frequency, underscoring the need for systematic screening against template vector integration following genome editing. Källstig et al. 2026

Image Credit: Evelyne Ruchti and Brian McCabe

https://doi.org/10.1371/image.pgen.v22.i07.g001