Figures
Self-cleaving ribozyme to control endogenous mRNA levels in a vertebrate model
Controlling mRNA and protein levels with genetic tools is essential for interrogating gene function. Here, we present a novel tool to modulate mRNA cleavage in zebrafish using a self-cleaving ribozyme. This brightfield image displays 36 hours post-fertilization zebrafish embryos, with wild-type pigmentation on top and pigmentation disrupted by the knockin of the T3H48 self-cleaving ribozyme at the slc45a2/alb locus below. The pigmentation phenotype recapitulates that observed in alb mutant animals. See Juan et al. Download February’s cover page.
Image Credit: Thomas Juan and Didier Y.R. Stainier
Citation: (2025) PLoS Genetics Issue Image | Vol. 21(2) April 2025. PLoS Genet 21(2): ev21.i02. https://doi.org/10.1371/image.pgen.v21.i02
Published: April 10, 2025
Copyright: © 2025 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Controlling mRNA and protein levels with genetic tools is essential for interrogating gene function. Here, we present a novel tool to modulate mRNA cleavage in zebrafish using a self-cleaving ribozyme. This brightfield image displays 36 hours post-fertilization zebrafish embryos, with wild-type pigmentation on top and pigmentation disrupted by the knockin of the T3H48 self-cleaving ribozyme at the slc45a2/alb locus below. The pigmentation phenotype recapitulates that observed in alb mutant animals. See Juan et al. Download February’s cover page.
Image Credit: Thomas Juan and Didier Y.R. Stainier