Figures
Y chromosome labelling in mitotic chromosomes from Anopheles gambiae testis
Y chromosome labelling in mitotic chromosomes from Anopheles gambiae testis. In this image, two Anopheles gambiae Y-linked satellite elements, AgY53B (Green) and AgY53A (Red), have been stained using DNA FISH on male gonial cell metaphase chromosomes. The CRISPR/Cas9 system was used to specifically target the satellite AgY53B during male meiosis, causing extensive damage to the Y chromosome. This damage results in Y-bearing sperm being unable to fertilize eggs while X-bearing sperm remain intact and viable. As a result, a strong bias toward female offspring is observed in the progeny of transgenic males. See Vitale et al. Download June’s cover page.
Image Credit: Matteo Vitale
Citation: (2024) PLoS Genetics Issue Image | Vol. 20(6) July 2024. PLoS Genet 20(6): ev20.i06. https://doi.org/10.1371/image.pgen.v20.i06
Published: July 9, 2024
Copyright: © 2024 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Y chromosome labelling in mitotic chromosomes from Anopheles gambiae testis. In this image, two Anopheles gambiae Y-linked satellite elements, AgY53B (Green) and AgY53A (Red), have been stained using DNA FISH on male gonial cell metaphase chromosomes. The CRISPR/Cas9 system was used to specifically target the satellite AgY53B during male meiosis, causing extensive damage to the Y chromosome. This damage results in Y-bearing sperm being unable to fertilize eggs while X-bearing sperm remain intact and viable. As a result, a strong bias toward female offspring is observed in the progeny of transgenic males. See Vitale et al. Download June’s cover page.
Image Credit: Matteo Vitale