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PLoS Genetics Issue Image | Vol. 16(6) July 2020

Flagellar mutants of the opportunistic bacterial pathogen Pseudomonas aeruginosa display nutrition-dependent rugose colony morphology.

Pictured is Pseudomonas aeruginosa strain AMT0113-12, an isolate from the sputum of a cystic fibrosis (CF) patient with chronic lung infection. This strain expresses a rugose colony phenotype on Vogel-Bonner Minimal Medium-based agar (shown here) that is not expressed on widely used lysogeny broth agar. This observation led to the discovery that flagellar mutations are linked to a similar gain-of-function (exopolysaccharide-overproducing) phenotype. While it has been assumed that a primary benefit from loss of motility is that the host immune system fails to target a bacterium lacking a flagellum, this observation suggests that exopolysaccharide-overproduction by flagellar mutants may also have fitness benefits in chronic infections and biofilms. See Harrison et al.

Download June's cover page.

Image Credit: Joe J. Harrison and Matthew R. Parsek.

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Flagellar mutants of the opportunistic bacterial pathogen Pseudomonas aeruginosa display nutrition-dependent rugose colony morphology.

Pictured is Pseudomonas aeruginosa strain AMT0113-12, an isolate from the sputum of a cystic fibrosis (CF) patient with chronic lung infection. This strain expresses a rugose colony phenotype on Vogel-Bonner Minimal Medium-based agar (shown here) that is not expressed on widely used lysogeny broth agar. This observation led to the discovery that flagellar mutations are linked to a similar gain-of-function (exopolysaccharide-overproducing) phenotype. While it has been assumed that a primary benefit from loss of motility is that the host immune system fails to target a bacterium lacking a flagellum, this observation suggests that exopolysaccharide-overproduction by flagellar mutants may also have fitness benefits in chronic infections and biofilms. See Harrison et al.

Download June's cover page.

Image Credit: Joe J. Harrison and Matthew R. Parsek.

https://doi.org/10.1371/image.pgen.v16.i06.g001