Figures
Tuning genetic circuitry to overcome a deleterious mutation
Zebrafish mef2ca mutants display variable craniofacial phenotypes. In this study, we showed that selective breeding for penetrance of mutant phenotypes can rapidly produce strains of fish that are highly sensitive to, or resilient against the deleterious mutation. Comparing the selectively bred strains reveals that artificial selection can change the genetic circuitry. Specifically, the downstream genes her6 and dlx5a are tuned so that the initial dysregulation is later corrected during patterning of the pharyngeal arches, shown here by in situ hybridization and the fli1a:EGFP transgene.
Image Credit: Raisa Bailon, University of Colorado Denver.
Citation: (2020) PLoS Genetics Issue Image | Vol. 15(12) January 2020. PLoS Genet 15(12): ev15.i12. https://doi.org/10.1371/image.pgen.v15.i12
Published: January 6, 2020
Copyright: © 2020 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Zebrafish mef2ca mutants display variable craniofacial phenotypes. In this study, we showed that selective breeding for penetrance of mutant phenotypes can rapidly produce strains of fish that are highly sensitive to, or resilient against the deleterious mutation. Comparing the selectively bred strains reveals that artificial selection can change the genetic circuitry. Specifically, the downstream genes her6 and dlx5a are tuned so that the initial dysregulation is later corrected during patterning of the pharyngeal arches, shown here by in situ hybridization and the fli1a:EGFP transgene.
Image Credit: Raisa Bailon, University of Colorado Denver.