Fig 1.
Outline of the major elements of forward genetics as envisaged by Ding and colleagues.
Proof of concept was achieved by mapping and characterising clog1. Coloured panels indicate different aspects of the study as follows: Violet panel—UV mutagenesis of protoplasts and screening for TS CLoG mutants. Yellow panels—NGM of clog1 mutation. Red panel—Validation of clog1. Blue panel—Bioinformatic search for CLoG1 orthologues in other plant groups. Green panels—Phenotypic interrogation of clog1. CLoG, conditional-loss-of-growth; NGM, next-generation mapping; NGS, next-generation sequencing; TS, temperature-sensitive; WT, wild-type.