Figure 1.
Bicyclus anynana linkage map and pan-macrolepidopteran synteny.
Orthologous B. anynana (empty) and Bombyx mori (dashed) LGs are shown with lines connecting orthologous markers. Numbering of B. anynana LGs reflects homology with B. mori (except for LG28). LGs labeled with “inv” were inverted to match marker order in the other species. Only those B. mori markers with mapped B. anynana orthologs are shown; their label follows the format A.B.C, with A = corresponding B. anynana marker name except for the “Ba” prefix, B = B. mori scaffold containing the marker, and C = log10(e-score) of the corresponding blast. Ordered markers are shown along the LGs (cM distances between B. anynana markers can be seen on scale to the right of each row of LGs), and unordered markers inside boxes. Marker names are shown in green if they correspond to visible mutations (likely position displayed as green shading inside the corresponding LG), blue if they either do not blast any B. mori scaffold or blast a B. mori scaffold which is not mapped, red if they blast a B. mori scaffold on a non-orthologous (i.e. different-number) B. mori LG, and black for all markers in orthologous B. mori LGs. Underlined B. anynana markers have mapped orthologs in Heliconius melpomene; nomenclature D.E.F for ordered markers follows: D = B. anynana marker name, E = H. melpomene linkage group [23] it blasts to, and F = log10(e-score) of the corresponding blast (same information for unordered markers is available in Table S4).
Figure 2.
See legend to Figure 1.
Figure 3.
See legend to Figure 1.
Figure 4.
See legend to Figure 1.
Table 1.
Summary of mapping information for the gene-based markers assigned to Bicyclus anynana linkage groups.
Table 2.
Visible mutants in mapping panel.
Figure 5.
Oxford Grid representing conservation of marker co-segregation between Bicyclus anynana and Bombyx mori linkage groups.
For each inter-specific LG pair, the number in the cell represents the total number of mapped orthologous markers (blast cut-off e-score 1.0e-05; numbers in brackets correspond to number of blast hits with e-scores greater that 1.0e-20). Grey shading highlights LG pairs sharing at least one orthologous gene.
Figure 6.
Mapped B. anynana pigmentation mutants.
Ventral surface of fore- (top) and hind-wing (bottom) of adult butterflies from different laboratory stocks: (A) “wildtype”, and mutants (B) Bigeye with all eyespots enlarged, (C) 067 with hindwing eyespots 6 and 7 enlarged, (D) Spotty with two extra eyespots on forewing, (E) Cyclops with fused eyespots, (F) Goldeneye with golden scales replacing the typically black scales of the eyespot mid-ring, and (G) Band with lighter distal wing half. Top view of a fifth and final instar larvae of different laboratory stocks: (H) “wildtype”, and (I) Chocolate mutant with dark-brown integument.
Table 3.
Color pattern loci in orthologous lepidopteran LGs.