Figure 1.
Effect of Champagne gene action on base coat colors of horses (chestnut, bay, and black).
A) Chestnut – horse only produces red pigment. B) Chestnut diluted by Champagne = Gold Champagne. C) Bay – black pigment is limited to the points (e.g. mane, tail, and legs) allowing red pigment produced on the body to show. D) Bay diluted by Champagne = Amber Champagne. E) Black – red and black pigment produced, red masked by black. F) Black diluted by Champagne = Classic Champagne.
Figure 2.
Champagne Eye and Skin traits.
A, B and C) Eye and skin color of foals. D and E) Eye color and skin mottling of adult horse.
Table 1.
Linkage Analysis between the Champagne Dilution and Microsatellite Markers; UM010, COOK007, TKY329 and VHL209.
Figure 3.
Example of Recombinant Haplotypes.
Linear relationship from top to bottom between the microsatellites, phenotype, and genotype of recombinant offspring for study sire #3. Phenotype is noted in top row with offspring's ID #.
Figure 4.
RT-PCR product results for SLC36A1, A2 and A3.
A) RT-PCR results for SLC36A1. B) RT-PCR results for SLC36A2. C) RT-PCR results for SLC36A3. (Faint bands observed above 400 bp on gel C were sequenced and did not show homology to SLC36A3.)
Figure 5.
Sequence Alignment and Gene Diagram.
Alignment is between homozygous champagne, non-dilute, and horse genome assembly. Reading frame is marked by alternating colors of codons. Bottom is diagram of SLC36A1 with the identified SNP in exon 2. Sequence and gene layout have been verified on Ensembl genome browser equine assembly v2. Blue blocks of gene layout are exons and red boxes are the 5′ and 3′ UTRs.
Figure 6.
Seven Species Protein Sequence Alignment for SLC36A1 exons 1 and 2.
The R highlighted in red is the amino acid replacement associated with the champagne phenotype.
Table 2.
Genotyping Results for c.188(C/G) locus.