Analysis of (p)ppGpp metabolism and signaling using a dynamic luminescent reporter
Fig 2
RsFluc reporter response to stimulation of (p)ppGpp synthesis.
Luminescence (RLU/OD600) of: A, RsFluc (black; JDB4496) and RsFlucmut (blue; JDB4631) after amino acid downshift at T60 (dashed line); B, RsFluc (black) and RsFlucmut (blue) after 0 and 60 mins of 50 ng/mL mupirocin treatment. Significance was determined by two-tailed t test: p-value < .05 for RsFluc, no significant difference for RsFlucmut; C, RsFluc grown in defined minimal media containing either 2.5 mg/mL of glucose (black) or 0.5 mg/mL glucose and 2.0 mg/mL arabinose (blue). Respective OD600 measurements are shown in grey and light blue, respectively, and D) RsFluc (black) and a PpurE luciferase promoter fusion in wildtype (orange; JDB4803) and (p)ppGpp0 (purple; JDB4804) backgrounds. Shown are representative examples of at least three biological replicates, each comprising three technical replicates.