Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source
Fig 2
Minimum integration rates and types of integration events as determined by fluorescence and PCR, respectively, for all injected constructs.
(A) Bar chart showing minimum integration rates which are calculated as follows: number of positive pools/total number of G0 survivors X 100%. Constructs with perfect homology arms are represented by open bars while those with recoded homology arms by filled boxes. P = plasmid. (B) Pie chart showing the proportion of canonical (black) to off-target/non-canonical (grey) integration events for each construct and their corresponding donor types. *N/A = Not applicable. PCR for these constructs were carried out on either pooled samples (n>5) or only one individual G1 from each pool (Table E in S1 File) rather than each individual positive G1.