MAPK-mediated transcription factor GATAd contributes to Cry1Ac resistance in diamondback moth by reducing PxmALP expression
Fig 8
Repressing PxMAP4K4 gene restores the expression of PxGATAd and PxmALP and susceptibility to Cry1Ac toxin.
(A) Effect of PxMAP4K4 gene silencing on PxGATAd and PxmALP expression. The expression of PxMAP4K4, PxGATAd and PxmALP was analyzed in the midgut tissue from larvae of NIL-R strain after different time periods post-injection with dsPxMAP4K4. The expression level (fold) of genes was normalized based on the value of the expression level detected at 0 h. (B) Susceptibility to Cry1Ac protoxin (LC10, 1000 mg/mL) in resistant NIL-R larvae injected with buffer, dsEGFP or dsPxMAP4K4. The mortality of untreated larvae was used as control. (A) and (B) The data are presented as mean values ± the SEM, the significance of the difference between values sets was calculated using Holm-Sidak’s test (*p < 0.05, **p < 0.01, ***p < 0.001, Holm-Sidak’s test, n = 3).