Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua
Fig 5
A cis-acting mutation in the promoter of CYP321A8 enhances the expression of this P450 gene in the resistant strain of S. exigua.
(A) Phylogenetic relationship of CYP321A8 promoter sequences obtained from the susceptible and resistant strains. The phylogeny was inferred by the maximum likelihood and percentage bootstrap values from 1000 replicates are displayed. (B) Analysis of the activity of the CYP321A8 resistant promoter (DNA5’-R) and susceptible promoter (DNA5’-S). Error bars display SD. Letters above bars denote a significant difference at p < 0.05 (ANOVA with post-hoc Tukey HSD). (C) The activity of progressive 5’ deletion constructs of the CYP321A8 promoter. Error bars display SD. Letters to the right of bars denote significant difference at p < 0.05 (ANOVA with post-hoc Tukey HSD). (D) The activity of progressive 5’-deletion constructs from -522 to -142 of the CYP321A8 promoter from the susceptible and resistant strains. Error bars display SD. Letters to the right of bars denote significant difference at p < 0.05 (ANOVA with post-hoc Tukey HSD).