Genome wide distribution of G-quadruplexes and their impact on gene expression in malaria parasites
Fig 2
Association of G4FS with genome features of P. falciparum.
(A) Fold-enrichment of G4FS in different genome features compared to 1,000 shuffling of G4H1.2 list. The dotted line indicates the fold-enrichment for the whole P. falciparum genome (value = 1.00). TSS: transcription start site. THSS: Tn5 hypersensitive sites. (B) Distribution profile of G4 motifs at threshold 1.2 around THSS peaks. The x-axis is centred on THSS peaks centre ± 1,000 bp. The red and blue lines correspond to G4FS on the coding and non-coding strands, respectively. (C) Boxplot representing the GC content in the different genomic features. The dotted line indicates the mean GC content of the P. falciparum genome. (D) Boxplot showing the G4 sequence length within promoters and in the whole genome. The mean sequence length (black point) of G4 within promoters was found significantly different from all G4 in the genome (two-tailed t-test, **p-value<0.01).