BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana
Fig 6
Residues in the protein kinase domain of IRE1A is required for non-canonical UPR gene expression in the BLI mutant background.
T-DNA mutant bli (bli-1) was crossed to either IRE1A mutant (ire1a) or IRE1B mutant (ire1b) to generate the respective double mutant plants. The wild-type form and various mutated forms of IRE1A were expressed in the bli ire1a double mutant background and 2-weeks-old T2 transgenic plants were collected for gene expression analysis. Fold change is the gene expression level in the mutants or the genetically complemented materials normalized to that in the WT, both of which were normalized to the expression of ACTIN. Error bars represent SE (n = 3). Asterisks indicate significance levels when comparing to the WT control in t-test. (*, p<0.05; **, p<0.01; n.s., not significant at p<0.05).