BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana
Fig 4
IRE1A mutation suppresses both canonical and non-canonical UPR gene expression in BLI mutant plants.
A-B, Gene expression analysis. T-DNA mutant bli (bli-1) was crossed to either IRE1A mutant (ire1a) or IRE1B mutant (ire1b) to generate the respective double mutant plants. Total RNA was exacted for RNA-Seq analysis (A) and qRT-PCR (B). Up-regulated or down-regulated genes in the mutants relative to the wild-type plants were used to draw the Venn diagrams. Fold change is the gene expression level in the mutants normalized to that in the WT, both of which were normalized to the expression of ACTIN. Error bars represent SE (n = 3). Asterisks indicate significance levels when comparing to the WT control in t-test. (*, p<0.05; **, p<0.01; n.s., not significant at p<0.05). bZIP60S, spliced bZIP60 transcript. Note that bZIP60-S, BiP3, NSF, DERD3A, SARA1A and NAC103 are up-regulated while MRN1 and LTP4 are not up-regulated by canonical ER stress inducer tunicamycin.