BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana
Fig 3
IRE1 mutations suppress the shoot growth phenotype of BLI mutant plants.
A-D, Phenotypic analysis. T-DNA mutant bli (bli-1) was crossed to either IRE1A mutant (ire1a) or IRE1B mutant (ire1b) to generate the respective double mutant plants. 2-weeks-old plants were photographed (A), weighted (B). Representative siliques were photographed (C) and siliques lengths were measurement at reproductive stage (D). Error bars represent SE (n = 3) in (B) and represent SD (n = 12) in (D). Letters above the bars indicate significant differences as determined by LSD test following ANOVA analysis (p< 0.05). Bar = 5 mm. E-F, Cell death and ROS accumulation analysis. 2-weeks-old plants were stained with trypan blue (E) or DAB (F). Arrows and arrow heads point to sites of cell death and ROS accumulation, respectively. Bar = 1 mm.