BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana
Fig 2
bZIP60 mutation suppresses UPR gene expression but not shoot growth phenotype of BLI mutant plants.
A-B, Phenotypic analysis. T-DNA mutant bli (bli-1) was crossed to either bZIP28 mutant (bzip28) or bZIP60 mutant (bzip60) to generate the respective double mutant plants. 2-week-old plant seedlings were photographed (A) and siliques lengths were measured later at reproductive stage (B). Error bars represent SD (n = 10). Letters above the bars in (B) indicate significant differences as determined by LSD test following ANOVA analysis (p<0.05). Bar = 5 mm. C, UPR gene expression analysis. Total RNA was exacted from 2-week-old plants for qRT-PCR. Fold change is the gene expression level in the mutants normalized to that in the WT, both of which were normalized to the expression of ACTIN. Asterisks indicate significance levels when comparing to the WT control in t-test. (*, p<0.05; **, p<0.01; n.s., not significant at p<0.05).