CDI/CDS system-encoding genes of Burkholderia thailandensis are located in a mobile genetic element that defines a new class of transposon
Fig 7
The IS2-like Translocatable unit mobilized to chromosome II in the 131–10 strain.
(A) Graphical representation of the region in chromosome 1 where the bcpAIOB-containing composite transposon is located (top). Graphical representation of strain 131–10 in which the entire composite transposon has been replaced by an nptII cassette (bottom). (B) PCR analyses to confirm removal of the MGE in strain 131–10 using primers P5 and P6 (shown as green arrows in panel A). PCR analyses detect DNA corresponding to genes within the composite transposon in strain 131–10. (C) IS2β5 in chromosome II in a WT background (top), or upon insertion of the IS2-like translocatable unit (bottom). PCR analyses of WT and strain 131–10 to confirm the insertion of the IS2-like translocatable unit into chromosome II. The distance between the binding site of primers β5-F and β5-R (grey arrows) is approximately 1.8 kb in WT DNA. Primers Circ1 and Circ2 (red arrows) bind specifically to the ends of the mobilized bcpAIOB-containing element. The insertion site of pABT73-TMP is denoted with a black arrowhead.