CDI/CDS system-encoding genes of Burkholderia thailandensis are located in a mobile genetic element that defines a new class of transposon
Fig 5
Megacircle production and community-associated behaviors, but not CDI, are disrupted in the absence of orfABβ.
(A) Models illustrating the possible correlations between CDS and megacircle formation. Delivered C-term of the BcpA toxin, dBcpA-CT. (B) Contact-dependent growth inhibition (CDI) is not affected by disruption of IS2α or IS2β. Inhibitors (WT, IS2α::nptII or IS2β::nptII) were co-cultured with ΔbcpAIOB (a strain susceptible to killing via CDI) for 24 hours then plated on selective media to calculate the competitive index (C.I.). When only WT bacteria were recovered, the data point is displayed in red; in this case the actual C.I. is greater than or equal to the represented value. Differences in C.I. are not statistically significant. (C) Ability of WT, IS2α::nptII or IS2β::nptII to display phenotypes linked to community-associated behaviors. These phenotypes include: production of pigments by colony biofilms (top), autoaggregation in M63 minimal medium (middle), or the proportion of Congo red (CR) binding colonies. P values were obtained using Mann-Whitney U test comparing mutant strains to WT. Results are shown as mean +/- SEM of three independent experiments (n = 6). **P < 0.01.