CDI/CDS system-encoding genes of Burkholderia thailandensis are located in a mobile genetic element that defines a new class of transposon
Fig 3
Megacircle production is disrupted in the absence of orfABβ.
(A) PCR analyses to detect the megacircle junction using primers Circ1 and Circ2 (top), or primers that bind within the putative composite transposon (bottom), in WT and strains with defective IS2α or IS2β elements. During construction of IS2α::nptII and IS2β::nptII, several kanamycin-resistant colonies were analyzed; a representative strain is shown. (B) Graphical representation of detection (or lack of) of a PCR product with primers Circ1 and Circ2 from an extrachromosomal megacircle (top) compared to detection of a product from the interphase of multiple copies of the element present in tandem repeats.