A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability
Fig 4
PDCD4-AS1 promotes the stability of PDCD4 mRNA.
A) PDCD4 and PDCD4-AS1 RNA level measured by RT-qPCR in control and PDCD4-AS1-depleted M1 cells. B) PDCD4 protein level in control and PDCD4-AS1-depleted M1 cells. C) PDCD4 and PDCD4-AS1 RNA level measured by qPCR in control and PDCD4-depleted M1 cells. D) PDCD4 protein level in control and PDCD4-depleted M1 cells. E) Nascent RNA assay in control and PDCD4-AS1-depleted M1 cells. F) RT-qPCR to quantify PDCD4 mRNA stability assay using RNA from control and PDCD4-AS1-depleted M1 cells treated with Flavopiridol (1M) for indicated time points (0, 1,2,3 and 6 hrs). Error bars in (A, D, E & F) represent mean ± SEM of N≥3 independent experiments (biological replicates). *P<0.05, ** P< 0.01 and ***P<0.001 using Student’s t test. N.S. represents not significant result.