A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability
Fig 3
PDCD4-AS1 negatively regulates cell proliferation and migration.
A-B) Transwell migration assay in control and PDCD4-AS1-depleted M1 cells. C-D) Wound healing assay in control and PDCD4-AS1 depleted M1 cells. Images were taken at Day 0, 1, 2 and 3 after wound creation; the magnified image of Day 3 in control and PDCD4-AS1 depleted cells is shown in the figure C. Ea,b) Transwell migration assay in M4 cells that are overexpressing PDCD4-AS1. Ec,d) Long-term anchorage-dependent plastic colony formation assay in M4 cells upon PDCD4-AS1 overexpression. F-G) Cell cycle flow cytometry in control and PDCD4-depleted M1 cells. H-I) Cell cycle flow cytometry in control and PDCD4-AS1-depleted M1 cells. J-K) Transwell migration assay in control and PDCD4-depleted M1 cells. L-M) Transwell migration assay in control and PDCD4-overexpressing M1 cells that are depleted of PDCD4-AS1 (AS-sh3). Error bars in (B, D, Eb, Ed, G, H, K & M) represent mean ± SEM of N≥3 independent experiments (biological replicates). *P<0.05, ** P< 0.01 and ***P<0.001 using Student’s t test. NS depicts not significant results.