A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability
Fig 2
PDCD4-AS1 is a stable nuclear-enriched lncRNA that shows concordant pattern of expression with its coding partner PDCD4.
A) Schematic representation of PDCD4/PDCD4-AS1 gene locus. B) PDCD4-AS1 RNA level measured by RT-qPCR in M1- M4 TNBC cells. C) PDCD4 mRNA level measured by RT-qPCR in M1- M4 TNBC cells. D) Immunoblot analysis shows the relative levels of PDCD4 protein in M1- M4 cells. E) Correlation analysis between PDCD4 and PDCD4-AS1 RNA in TCGA breast cancer dataset, analyzed by TANRIC platform. F) PDCD4-AS1 RNA level in different subclasses of breast cancer patients, analyzed by TANRIC platform. G) PDCD4-AS1 RNA level in different stages of breast cancer patients analyzed by TANRIC platform. H) Kaplan–Meier analysis to depict the survival rate in TCGA breast cancer patients with high and low levels of PDCD4-AS1, analyzed by TANRIC platform. I-J) RT-qPCR analyses in poly A+ and poly A- (I) and nuclear and cytoplasmic fractionated RNA (J) from M1 cells. K) RT-qPCR to quantify the stability of PDCD4-AS1 and PDCD4 mRNA using RNA from M1 cells treated with Flavopiridol (1M) for indicated time points (0, 1,2,4 and 6 hrs). Error bars in (B, C, J & K) represent mean ± SEM of N≥3 independent experiments (biological replicates). *P<0.05, ** P< 0.01 and ***P<0.001 using Student’s t test.