Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells
Fig 9
Summary of experimental results for tmie constructs and model of discrete functional domains of Tmie.
(A) Symbols as follows: enhanced above wild type (++), comparable to wild type (+), partially reduced (+/-), severely reduced or absent (-), and not applicable (n). For dominant negative effect, the effect is present (+) or absent (-). Blank spaces were determined for reasons discussed in the text. Refer to Fig 5A for details on constructs. (B) A model of the protein sequence of zebrafish Tmie. Amino acids 1–43 are separated due to suspected cleavage as a signal peptide. Although shown as a TM domain, it is unclear whether the first hydrophobic region forms a helix. Note that the extracellular region (yellow glow) was never deleted in its entirety; SP63-231 deleted QIPDPELLPTDPPKKPDPV, and Δ63–73 deleted TSETVVFWGLR. Also note that the TM domain (orange lettering) only had an effect on the stability of Tmie when the entire helix was substituted.