Cyc8p and Tup1p transcription regulators antagonistically regulate Flo11p expression and complexity of yeast colony biofilms
Fig 5
Extracellular fibrillar material between cells within colonies of strains with altered levels of Cyc8p and Tup1p.
A, Electron microscopy of Velcro connections between cells from 3-day-old (a, b, e) and 5-day-old (c, d) colonies grown on GMA. B, The average length of the fibers is shown in the graph. Black columns, structured colony biofilms; white columns, smooth colonies; bars, SD; ***, P<0.001. C, Examples of extracellular material in higher magnification from colony biofilms (BR-F, inset a1 from Aa) and from smooth colonies (tup1, inset d1 from Ad). Velcro-like connections in BR-F are schematically indicated in yellow color, the red color indicates N-terminal Flo11A domains [48], potentially involved in the interaction.