Cyc8p and Tup1p transcription regulators antagonistically regulate Flo11p expression and complexity of yeast colony biofilms
Fig 2
Expression of Flo11p adhesin in colonies with altered levels of Cyc8p and Tup1p.
A, Effects of TUP1 (left) and CYC8 (right) expression induced by galactose diffusing from the wells in the agar (marked in red) on colony morphology. 120 μl of 10% galactose was applied to each well. Morphologies of wt colonies treated in the same way are shown in S5 Fig. B, Flo11p-GFP in 3-day-old colonies of Flo11p-GFP strains grown on GMA. Representative result of the 4 experiments is shown. Western blot loading controls are in S4 Fig. C, 2PE-CM of cross-sections of 3-day-old colonies of BR-F-Flo11p-GFP strain grown on GMA. Green, Flo11p-GFP; red, cell autofluorescence (in upper picture). Plating density ~4–6 x 103 colonies per plate. D, 2PE-CM of cross-sections of colonies from the plates with galactose induction (panel A). Induced colonies localized close to the galactose source and uninduced colonies localized to the plate margin are shown. Green, Flo11p-GFP; red, cell autofluorescence. Plating density ~4–6 x 103 colonies per plate.