OFF-responses of interneurons optimize avoidance behaviors depending on stimulus strength via electrical synapses
Fig 6
Omega turns are correlated with strong and broad calcium mobilization, and lin-32 and inx-1 mutants show impaired calcium mobilization.
(A) Heat maps of calcium imaging of the neck muscles in vivo. Arrows indicate the area with high calcium. (B) Scatter plot of the maximum calcium fluorescence changes in individuals. The values are higher for omega turns than for reversals. **p < 0.01, ANOVA followed by the Tukey's post hoc tests. n = 24,18,17. (C) Scatter plot of the areas of the calcium increase in individuals. Areas are broader for omega turns than for reversals. ***p < 0.001, ANOVA followed by the Tukey's post hoc tests. n = 23,20,21. (D) Random samples of lin-32 and inx-1 mutants show tendency of impaired calcium fluorescence changes. *p < 0.05, ANOVA followed by the Tukey's post hoc tests. n = 20 in each strain. (E) Random samples of lin-32 and inx-1 mutants showed tendency of decreased calcium-induced areas. *p < 0.05, ANOVA followed by the Tukey's post hoc tests. n = 20 in each strain. n means the number of individuals (animals). Plots represent the mean ± SD.