RES complex is associated with intron definition and required for zebrafish early embryogenesis
Fig 5
Molecular features that determine intron retention in RES are associated with splicing through intron definition (A-G). Box plots showing the median (black solid line) and the distribution of values for multiple intron-exon features of several groups of introns of interest. “RESdep”, all highly retained introns (∆PIR >15) in at least two out the three mutants in the RES complex; “no-NMD”, subset of “RESdep” retained introns that are predicted not to trigger NMD; “NMD”, subset of “RESdep” retained introns predicted to trigger NMD; and “Ctr”, control set of introns with a ∆PIR cutoff < 0.5 in the three RES complex mutants (see Materials and Methods for details). (*** P ≤ 0.001; Mann-Whitney-U test). Branch point (BP) definition: BP Score of best-predicted BP. Scored base on Corvelo et al. [24] (See Materials and Methods for details).