FMRFa receptor stimulated Ca2+ signals alter the activity of flight modulating central dopaminergic neurons in Drosophila melanogaster
Fig 7
Dopaminergic neurons require synaptic vesicle recycling for sustained flight.
(A) Acute expression of a temperature sensitive dynamin mutant (Shits) in adult dopaminergic neurons reduced flight bout durations when tested at 30°C as compared to a control tested at 22°C (THD1>Shits at 22°C compared to 30°C; n≥30, **p<0.01, Mann-Whitney U-test). THD1/+ and Shits/+ controls in the two temperature conditions are also shown. (B) Adult specific knockdown of the dopamine synthesizing enzyme, Tyrosine Hydroxylase (TH) by expressing an RNAi (THRNAi) in dopaminergic neurons significantly reduces flight duration as compared to the corresponding 18°C controls (THD1;TubGAL80ts>THRNAi; n≥30, **p<0.01, Mann-Whitney U-test).