FMRFa receptor stimulated Ca2+ signals alter the activity of flight modulating central dopaminergic neurons in Drosophila melanogaster
Fig 5
Expression of FMRFaRRNAi and inhibition of CaMKII in dopaminergic neurons reduces calcium entry after a depolarizing stimulus.
(A) Mean trace (±SEM) of normalized GCaMP6m responses observed upon KCl stimulation (green arrow) in 8 day adult neurons in the three indicated genotypes, THD1;TubGAL80ts>GCaMP6m, Control in black; THD1;TubGAL80ts>GCaMP6m;FMRFaRRNAi, in red and THD1;TubGAL80ts>GCaMP6m;Ala, in blue. (B) Area under the curve and (C) Peak change in fluorescence quantified from (A). Each bar is compared to control shown in black (**p<0.01; Mann-Whitney U-test). (D) Snap shots of GCaMP6m responses from the same genotypes shown in (A). In the FMRFaRRNAi knockdown condition (2nd row), the yellow arrow indicates a cell that responded whereas pink arrows indicate cells with no response or a minimal response to the depolarization stimulus. Scale bars represent 50 μm.