FMRFa receptor stimulated Ca2+ signals alter the activity of flight modulating central dopaminergic neurons in Drosophila melanogaster
Fig 2
FMRFaR is expressed on dopaminergic neurons and is functionally active.
(A) Quantitative PCR on FACS sorted dopaminergic (GFP +ve, TH) and non-dopaminergic (GFP–ve, non TH) neurons, shows enrichment of FMRFaR transcripts in dopaminergic neurons. Each bar represents normalized fold change as mean ± SEM (n≥4, *p<0.05, unpaired t-test) (B) Snap shots of GCaMP6m responses from the indicated genotypes. The red box represents the magnified region of the protocerebrum shown in subsequent images. Warmer colors denote increase in [Ca2+]. Scale bars indicate 20 μm. (C, D) Mean traces (±SEM) of normalized GCaMP6m fluorescence responses (ΔF/F) in THD1GAL4 marked cells upon peptide or solvent addition (green arrow). (E) Area under the curve and (F) Peak ΔF/F quantified from 0 s to 420 s in (C, D). The box plot limits extend from 25th to 75th percentile. The line and solid diamond represent the median and mean respectively. Individual data points are shown as open diamonds. Numbers below indicate total number of cells imaged. Each bar is compared to the Control peptide stimulated condition shown in black (**p<0.01; ns—not significant; Mann-Whitney U-test).