Reduced monocyte and macrophage TNFSF15/TL1A expression is associated with susceptibility to inflammatory bowel disease
Fig 6
Monocyte-derived macrophages also favor expression of the IBD-protective allele.
(A) Peripheral blood monocytes from rs6478109 heterozygotes were differentiated into macrophages in the presence of M-CSF (n = 10) or (B) GM-CSF (n = 7) before examining allele-specific expression. (C) Macrophages derived in (A) were stimulated with LPS for 4 hours before examining allele-specific expression (n = 10, genomic DNA as for panel (A)). ASE was measured at rs4263839 (ratio of A/G reported) in the first intron of TNFSF15. All individuals were heterozygous at both rs4263839 and rs6478109. rs4263839:A is in phase with the IBD-protective allele rs6478109:A in 1000 Genomes EUR subjects.