Systematic identification of factors mediating accelerated mRNA degradation in response to changes in environmental nitrogen
Fig 4
BFF estimates of GAP1 mRNA abundance per mutant.
A) Flow cytometry analysis of GAP1 mRNA abundance in the prototrophic deletion collection (n = 3,230 mutants) before and after the upshift. The vertical gray lines denote boundaries of the four FACS gates. Biological replicates are indicated by color. B) Measurements for individual genes before and after the upshift. Pseudo-events per strain per bin are on the y-axis. Black dashed lines indicate maximum-likelihood fits of a log-normal to pseudo-events within each bin for each mutant. For plotting purposes, points are positioned on the x-axis at the average signal for the library in that bin. Colors are as in panel A. C) Distribution of modeled mean GAP1 mRNA levels for each mutant. D) The mean GAP1 mRNA expression levels fit using all replicate data for individual mutants before and after the upshift are shown as points connected by a line, colored according to the type of gene. The background violin plot shows the distribution of all 3,230 mutants. Plots for all mutants are available in the associated Shiny application (Methods).