Systematic identification of factors mediating accelerated mRNA degradation in response to changes in environmental nitrogen
Fig 3
GAP1 mRNA dynamics measured by flow cytometry.
A) GAP1 mRNA following upshift measured using RT-qPCR, relative to an external spike-in mRNA standard. The dashed line is fit to points 2 minutes after the upshift. B) Flow cytometry of wild-type yeast probed for GAP1 mRNA in nitrogen-limited conditions and following an upshift. The vertical grey lines indicate FACS gate boundaries used for cell sorting. C) Representative cells from each bin sorted from the experiment in panel B. D) Quantification of microscopy data. Each black dot represents a single cell. The mean number of foci per cell in each bin from panel B is displayed as a red point.