Non-equilibrium repressor binding kinetics link DNA damage dose to transcriptional timing within the SOS gene network
Fig 2
Effect of UV dose on the timing of peak promoter activity.
A. Promoter activity for the uvrD and sulA promoters plotted as a function of time after a range of UV doses. Promoter activity (PA) measurements were acquired at 3 minute intervals. Solid lines and shading of the same color connect data points and indicate the mean and standard error of promoter activity measurements, respectively. Colors indicate UV dose, as shown in the figure legend. Horizontal dotted lines indicate the value of 90% of peak promoter activity (0.9*PApeak) for that trace and vertical dotted lines indicate the time at which 0.9*PApeak occurred (tpeak). B. tpeak plotted as a function of UV dose. Promoters were binned into early (blue), middle (red), or late (black) time categories based on tpeak values at 100 J/m2. Lines connecting data points from each UV dose are shown for ease of visualization.