Auxin production in diploid microsporocytes is necessary and sufficient for early stages of pollen development
Fig 6
Localized auxin production in microsporocytes and microspores rescued the pollen defects in yuc2yuc6 mutants.
The green color indicates the expression pattern of the ARF17 (A) and LAT52 (B) promoter. The ARF17 promoter is known to be active in microsporocytes and microspores [63]. LAT52 is expressed in microspore and gametophyte specifically. Msp, Microsporocytes; Mp, microspores; Bc, Bicellular pollen; Tc, Tricellular pollen. Morphology of adult shoots (Bars = 2 cm), Alexander staining (Bars = 100 μm) of anthers and DAPI staining (Bars = 10 μm) of pollens from ProARF17:YUC2-GFP (yuc2yuc6) (A) and ProLAT52:YUC2-GFP (yuc2yuc6) (B) transgenic plants. Note that YUC2-GFP expression driven by ARF17 and LAT52 promoters are sufficient to rescue the yuc2yuc6 defects. In situ hybridization of GFP (Bars = 20 μm) and fluorescence images (Bars = 100 μm) of the YUC2-GFP fusion protein in anthers from yuc2yuc6 transformed with ProARF17:YUC2-GFP (A) and ProLAT52:YUC2-GFP (B). In ProARF17:YUC2-GFP (yuc2yuc6) anther, GFP is expressed in microsporocytes and in microspores at early stage (A). In ProLAT52:YUC2-GFP (yuc2yuc6) anther, GFP is significantly expressed in microspores at late stage and in bicellular pollens (B). TS, Tricellular Stage; BS, Bicellular Stage; US; Unicellular Stage.