SHOC1 is a ERCC4-(HhH)2-like protein, integral to the formation of crossover recombination intermediates during mammalian meiosis
Fig 5
Shoc1 DNA binding specificity.
(A) Schematic showing conserved motifs within human SHOC1570-1111. (B) Shoc1 purification protocol and Coomassie stained SDS-PAGE showing purified SHOC1570-1111. SHOC1570-1111 was purified to near homogeneity after Dextrin (1), Mono Q/S (2), and Superdex 200 (3) chromatography. (C) Representative native PAGE gel shift assays showing SHOC1570-1111 binding to distinct DNA substrates. (D) Quantitation of Shoc1 binding to DNA substrates shown in C (n = 2–3, mean ± SD). (E) Deletion of the (HhH)2 domain reduces human SHOC1 binding to single-stranded DNA. Schematic diagram of the SHOC1570-1041 mutant and structural characteristics of (HhH)2 domains of human XPF, SHOC1 and MUS81 are also shown. Coomassie stained SDS-PAGE showing purified SHOC1570-1041 is also shown. Mono Q/S (1), and Superdex 200 (2) chromatography.