Dynamics and control of sister kinetochore behavior during the meiotic divisions in Drosophila spermatocytes
Fig 5
Behavior of KTs of univalents in mnm mutants during M I.
After time lapse imaging with spermatocyte cysts from mnm mutant flies expressing Cid-EGFP and His2Av-mRFP, all eight KTs were tracked and assigned to chromosomes. (A) VKT values plotted over time from a representative spermatocyte. Onset of the indicated phases marked by dotted lines. Metaphase onset was scored when the rapid KT jumps vanished rather than after bi-orientation of the last bivalent as in controls. (B) Comparison of KT tracks in control (left) and mnm mutants (right). The track of one representative KT from NEBD until metaphase onset is displayed as an overlay on the still frame from metaphase onset. Track color corresponds to time from early (blue) to late (red). White dotted lines demarcate the metaphase plate region (width 3 μm corresponding to about ¼ of the pole-to-pole distance). Scale bar = 3 μm. (C) State of sister KT conjunction during M I after Spc105 depletion in spermatocytes with and without mnm and snm function. The normal number of eight Cid-EGFP dots was detected by time lapse imaging with spermatocytes from the indicated genotypes. Still frames from representative spermatocytes during early prometaphase I are displayed. Scale bar = 5 μm.