Patterns of chromatin accessibility along the anterior-posterior axis in the early Drosophila embryo
Fig 6
Single nuclei ATAC-seq from cellular blastoderm embryos largely agrees with data from embryo halves.
Recently published single nuclei ATAC-seq data [50] corresponding to the cellular blastoderm were separated into anterior and posterior groups following designations determined by [50]. (A) Genome browser trace at the eve locus. Whole embryos, anterior halves, and posterior halves are in grey, orange, and blue respectively. Merged anterior single nuclei and merged posterior single nuclei from [50] are shown in peach and grey blue or cesious. Finally, single anterior and single posterior halves from our study are shown in rusty red and light blue. DnaseI hypersensitivity data is in green. Peaks are depicted in bars below the pooled halves and whole data. Light gray bars denote the gene annotation while the black bars denote annotated enhancers. Colored annotation bars represent enhancers analyzed in Fig 3. (B) Positional skew scores calculated for single nuclei and halves ATAC-seq data at all A-P and D-V patterning regions (enhancers and promoters) are plotted with halves on X and single nuclei on Y. Dotted line indicates X = Y. (C) Bar graph shows the positional skew scores calculated from single nuclei ATAC-seq data at all anterior (orange) and posterior (blue) patterning enhancers in the dataset. Asterisks denote enhancers whose accessibility skew scores show statistical significance over random regions (p < 0.05). (D) Bar graph shows the positional skew scores calculated from single nuclei ATAC-seq data at all anterior (orange) and posterior (blue) patterning promoters in the dataset. Asterisks denote promoters whose accessibility skew scores show statistical significance over random regions (p < 0.05).